339194

antibody from Invitrogen Antibodies

Targeting: TJP1 DKFZp686M05161, MGC133289, ZO-1

Provider product page for 339194

Western blot

ELISA

Immunocytochemistry

Other assay

Antibody data

Antibody Data
Antigen structure
References [22]
Comments [0]
Validations
Immunocytochemistry [2]
Other assay [10]

Submit

Product number: 339194 - Provider product page
Provider: Invitrogen Antibodies
Product name: ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 594
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Reactivity: Human, Canine
Host: Mouse
Conjugate: Red dye
Isotype: IgG
Antibody clone number: ZO1-1A12
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: 4° C, store in dark

TJP1 protein structure - 339194 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: NULL

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 6. Hox4 proteins are necessary and sufficient to induce non-autonomous apical cell enlargement. (A-H) Confocal immunostaining for Hoxb4 (green) and ZO-1 (red) on E10.5 flat-mounted mouse hindbrains of wild-type ( Hoxb4 +/+ ; Hoxd4 +/+ ) (A,B), Hoxb4 +/+ ; Hoxd4 -/- (D), Hoxb4 +/- ; Hoxd4 -/- (F) and Hox4 double-mutant (H,I) embryos at a lateral level of the r6/r7 boundary region. The r6/r7 boundary or its presumptive position (x) are indicated in A and H. (B,I) Confocal z-projections of similar magnifications of the r6/r7 boundary region as in D (corresponding to dotted boxes in A and H) showing the apical cell outlines (yellow) used for quantitation. (C,E,G,J) Apical areas (mum 2 ) with respect to the anteroposterior position of the cells in B,D,F,I, respectively. Hoxb4 + (green) and Hoxb4 - (black) cells are indicated. (K,L) Confocal z -projections and ZO-1 apical outlines (yellow) of the chick r5 neuroepithelium electroporated with mouse Hoxb4-mGFP . (K) Mouse Hoxb4/GFP + cells (green) form a cluster with a well-defined interface (dotted line). (L) Corresponding apical cell areas (mum 2 ) with respect to anteroposterior position for mouse Hoxb4 + (green) and mouse Hoxb4 - (black) cells. Anterior is towards the left.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 1 a-c Quality control steps for the cultivation of Caco-2 cells on filter inserts. Increase in TEER over the cultivation period ( a ). Caco-2 cells were seeded onto filter inserts applying Protocol #2 and TEER was measured as detailed in Supplementary Protocol #3 (Additional file 1 ). The data points represent mean +- SD from three inserts from the respective plate. Representative image of a Caco-2 cell monolayer grown on a filter insert for 21 days ( b ). Depicted is a maximum projection of the top view and the optical cross sections obtained by CLSM. Cell nuclei are shown in blue (DAPI) and the tight junction protein ZO-1 in red (Alexa Fluor 594 conjugated anti ZO-1 antibody). Staining was carried out as described in Protocol #6. Dextran Blue permeability of a filter insert without cells and a filter insert with a fully differentiated cell monolayer ( c ). Filter inserts were incubated with Dextran Blue as described in Supplementary Protocol #4 (Additional file 1 ) over a period of 6 h and sampling every hour. The data points represent mean +- SD from three to four independent experiments

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 Integrity of ARPE-19 cells evaluated by zonula occludens-1 (ZO-1) (red) and caspase-3 (green) immunofluorescence. VITD (1 nM, 1 h; ( D )) did not affect ZO-1 structure compared to saline ( A , G ). Lipopolysaccharide (LPS) ( B , H ) and H 2 O 2 ( E , K ) addition damaged tight junctions and concomitant incubation with VITD (1 nM, 1 h; ( C , I ) and ( F , L )) restored the altered structure. ( G-L ) show the apical junction in higher magnification. Caspase-3 was highly observed in the H 2 O 2 group ( E ) compared to saline ( A ) and VITD ( D ). VITD addition showed restoration, and caspase-3 activation was absent ( F ). Nuclei were labeled with DAPI (blue). Scale bar: 20 um. Densitometry of ZO-1 expression in ARPE-19 cells under oxidative stress ( M ) and inflammatory ( N ) conditions. Although a tendency to reduce the ZO-1 expression was observed, no statistical differences were found. VITD restored values similar to saline group. n = 3.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 1 Junctional integrity of ARPE-19 cells evaluated by ZO-1 (red) fluorescence under a confocal imaging system. Application of N and ND treatments (62.34 mug/mL each) did not affect tight junctions, cell integrity, and structure ( B , C ) compared to saline ( A ). Addition of H 2 O 2 (2h; 1600 muM) and LPS (24 h; 20 mug/mL) to induce oxidative stress and inflammatory-like conditions, respectively, damaged tight junctions ( D , G ), while incubation with N and ND treatments during the last hour of the induction recovered the altered structure ( E , F , H , I ). Nuclei were labelled with DAPI (blue). Scale bar: 20 um.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 2 RPE flatmounts are viable for at least one week after culture in vitro . ( A ) RPE flatmounts were prepared from 2- and 9-month-old wild-type C57BL/6J mice and cultured in complete medium with or without H 2 O 2 for up to one week. Protein lysates were prepared from the RPE flatmounts and subjected to Western blotting analysis. The cell death marker RIP1 was used to evaluate cell viability; vinculin was used as an internal control. As a positive control: RPE flatmounts treated with either 1 mM or 10 mM H 2 O 2 had significant expression of RIP1. Further, the RPE flatmount that was cultured for 7 days without changing the medium (mishandled sample) showed a slight amount of RIP1, whereas the RPE flatmounts cultured with frequent medium changes did not express RIP1, suggesting that the RPE cells obtained even from older mice (9 months) were viable after at least 7 days of culture. ( B ) RPE flatmounts were obtained from 3-month-old wild-type C57BL/6J mice and cultured in complete medium with or without H 2 O 2 treatment for up to one week. RPE flatmounts were then fixed in 2.5% glutaraldehyde and processed for transmission electron microscopy (TEM). The TEM imaging of the RPE flatmounts cultured for 16 h, 3 days, and 7 days showed that these RPE cells have normal nuclei (yellow arrows) and organelles, such as mitochondria (yellow arrowheads), and abundant microvilli. H 2 O 2 -treated RPE flatmounts showed cell death symptoms, including chromatin condensation and fragme

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 6 Detection of differentiated cells in NHBE pseudostratified epithelial layer generated from passage 1 cultures. Immunofluorescence for ( A ) Ciliated cells (alpha-tubulin, green), ( B ) goblet cells (MUC5B, green), ( C ) goblet cells (MUC5AC, red), ( D ) ZO-1 tight junctions (red), nuclear stain (DAPI - blue); and staining for ( E ) periodic acid-Schiff/alcian blue for mucus producing goblet cells.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 5 Immunocytochemical staining of tight junction proteins in cerebral endothelial cells with different paracellular permeability. White arrows indicate disrupted alignment of tight junction proteins. Scale bars, 10 mum.