R31647

antibody from NSJ Bioreagents

Targeting: TJP1 DKFZp686M05161, MGC133289, ZO-1

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

R31647

Provider

NSJ Bioreagents

Product name

ZO-1 Antibody

Antibody type

Polyclonal

Antigen

Human partial recombinant protein (AA 1178-1527) was used as the immunogen for this ZO-1 antibody.

Description

Antigen affinity purified antibody

Reactivity

Human, Rat

Host

Rabbit

Conjugate

Unconjugated

Vial size

100 µg

Concentration

Lyophilized; resuspend with 200 ul for 0.5 mg/ml

Storage

After reconstitution, the ZO-1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of ZO-1 antibody and Lane 1: rat liver; 2: mouse liver; 3: (r) NRK; 4: (r) PC12; 5: human HeLa; 6: (h) SMMC-7721; 7: (m) HEPA; 8: (h) COLO320 lysate. Predicted/observed ~194KD.

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

Western blot testing of ZO-1 antibody and recombinant human protein (0.5ng)

Immunohistochemistry

Supportive validation

Submitted by

NSJ Bioreagents (provider)

Main image

Experimental details

IHC-P: ZO-1 antibody testing of human intestinal cancer tissue. HIER: steamed with pH6 citrate buffer.