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ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Immunocytochemistry [1]
- Other assay [1]
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- Product number
- MA3-39100-A555 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ZO-1 Monoclonal Antibody (ZO1-1A12), Alexa Fluor™ 555
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human, Canine
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- ZO1-1A12
- Vial size
- 50 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C, store in dark, DO NOT FREEZE!
Submitted references Age-Related Differences in Structure and Function of Nasal Epithelial Cultures From Healthy Children and Elderly People.
Single-cell-resolved differentiation of human induced pluripotent stem cells into pancreatic duct-like organoids on a microwell chip.
Balázs A, Millar-Büchner P, Mülleder M, Farztdinov V, Szyrwiel L, Addante A, Kuppe A, Rubil T, Drescher M, Seidel K, Stricker S, Eils R, Lehmann I, Sawitzki B, Röhmel J, Ralser M, Mall MA
Frontiers in immunology 2022;13:822437
Frontiers in immunology 2022;13:822437
Single-cell-resolved differentiation of human induced pluripotent stem cells into pancreatic duct-like organoids on a microwell chip.
Wiedenmann S, Breunig M, Merkle J, von Toerne C, Georgiev T, Moussus M, Schulte L, Seufferlein T, Sterr M, Lickert H, Weissinger SE, Möller P, Hauck SM, Hohwieler M, Kleger A, Meier M
Nature biomedical engineering 2021 Aug;5(8):897-913
Nature biomedical engineering 2021 Aug;5(8):897-913
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ZO-1 (orange) in Caco-2 cells. The cells were fixed with 4% Paraformaldehyde in PBS for 15 minutes at room temperature, and blocked with 3% BSA in PBS (Product # 37525) for 30 minutes at room temperature. Cells were stained with a ZO-1 Monoclonal Antibody, AlexaFluor 555 conjugate (Product # MA3-39100-A555) at a dilution of 5 µg/mL in blocking buffer for 1 hour at room temperature protected from light. Nuclei (blue) were stained with Hoechst Dye (Product # 62249) at a dilution of 1:10,000 in blocking buffer. Images were taken on a Thermo Scientific ToxInsight Instrument at 20X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 3 Apical-out polarity of the microwell-chip-derived PDLOs switched upon orthotopic transplantation or embedding into Matrigel. a , Schematic of the Matrigel and orthotopic transplantation experiment. PDLOs were transplanted on day 27 and mice were sacrificed after 8 weeks. b , Overview haematoxylin-eosin (HE) staining and magnification of the engraftment site depicted by the dashed square (n=2 mice). Scale bar: 500 mum for overviews, 50 mum for magnification. c , PDLOs formed human epithelial duct-like tissue in vivo . (H-NUCL: human-specific nucleoli). Scale bar denotes 50 mum. d , IF images for the apical markers ZO1 and AcTUB and basal markers COL4A1 and ARL13B on 3D pancreatic-progenitor aggregates, PDLOs, Matrigel PDLOs and transplanted PDLOs. Complementary images are depicted in Supplementary Fig. 5 . The nuclei were counterstained with DAPI. Scale bar denotes 50 mum.
