- PA5-19090 - Invitrogen Antibodies TJP1 antibody

Submitted references A look into retinal organoids: methods, analytical techniques, and applications.

Afanasyeva TAV, Corral-Serrano JC, Garanto A, Roepman R, Cheetham ME, Collin RWJ
Cellular and molecular life sciences : CMLS 2021 Oct;78(19-20):6505-6532

Annexin A1/Formyl Peptide Receptor Pathway Controls Uterine Receptivity to the Blastocyst.

Hebeda CB, Sandri S, Benis CM, Paula-Silva M, Loiola RA, Reutelingsperger C, Perretti M, Farsky SHP
Cells 2020 May 11;9(5)

Development of a miniaturized 96-Transwell air-liquid interface human small airway epithelial model.

Bluhmki T, Bitzer S, Gindele JA, Schruf E, Kiechle T, Webster M, Schymeinsky J, Ries R, Gantner F, Bischoff D, Garnett J, Heilker R
Scientific reports 2020 Aug 3;10(1):13022

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Western Blot staining of Human Cerebral Cortex lysate using Product # PA5-19090 at a concentration of 0.3 µg/mL, the primary antibody incubation was 1 hour and the detection method was chemiluminescence.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Immunofluorescence analysis of ZO-1 was performed using 90% confluent log phase Caco-2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ZO-1 Rabbit Polyclonal Antibody (Product # PA5-19090) at 5 µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Rabbit anti-Goat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11078) at a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing junctional localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Knockout of ZO-1 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, AssayID CRISPR533979_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Immunofluorescence analysis was performed on wild type Caco-2 cells (panel a,d), Caco-2 Cas9 control cells (panel b,e) and Caco-2 ZO-1KO cells (panel c, f). Cells were fixed, permeabilized, and labeled withZO-1 Polyclonal Antibody (Product # PA5-190908203, 3 µg/mL dilution), followed by Donkey anti-Goat IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32814, 1:2000). Nuclei (blue) were stained using ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962), and Rhodamine Phalloidin (Product # R415, 1:300) was used for cytoskeletal F-actin (red) staining. Loss of signal (panel c,f) upon CRISPR mediated knockout (KO) confirms that antibody is specific to ZO-1 (green). The images were captured at 60X magnification.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 3 AnxA1 controlled claudin-1 and ZO-1 expressions on uterine epithelial cells via FPR1 and FPR2. Claudin-1 ( A ) and ZO-1 ( B ) expressions on uterine epithelial cells were determined 24 h after incubations. Representative images of claudin-1 and ZO-1 immunofluorescence are shown. (-) means absence and (+) means presence of treatments. The data are expressed as mean +- standard error of mean of three to five independent experiments. a p < 0.05 vs. NT; b p < 0.01 and c p < 0.05 vs. AnxA1.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Figure 4 AnxA1 controlled Muc-1 and Claudin-1 expressions on uterine epithelial cells via ERK1/2 phosphorylation. The effect of AnxA1 on ERK ( A ) and STAT1alpha ( B ) phosphorylation and NF-kappaB expression ( C ) were investigated by flow cytometry. The inhibition on ERK1/2, evoked by PD98059 incubation, was investigated on Muc-1 ( D ), Claudin-1 ( F ) and ZO-1 ( H ) expressions using immunofluorescence. Representative images of Muc-1, Claudin-1 and ZO-1 are shown in ( E ), ( G ) and ( I ), respectively. (-) means absence and (+) means presence of treatments. The data are expressed as mean +- standard error of mean of three to five independent experiments. a p < 0.05 and b p < 0.01 vs. NT; c p < 0.05 and d p < 0.01 vs. AnxA1.

PA5-19090

Antibody data