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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
- Immunohistochemistry [1]
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- Product number
- H00054487-M01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00054487-M01, RRID:AB_1112525
- Product name
- DGCR8 monoclonal antibody (M01), clone 4G3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant DGCR8.
- Antigen sequence
LASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQE
TSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAE
EREETRKKPKMSIVASAQPGGEPLCTV- Isotype
- IgG
- Antibody clone number
- 4G3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DGCR8 monoclonal antibody (M01), clone 4G3. Western Blot analysis of DGCR8 expression in human spleen.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of DGCR8 expression in transfected 293T cell line by DGCR8 monoclonal antibody (M01), clone 4G3.Lane 1: DGCR8 transfected lysate (Predicted MW: 86 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged DGCR8 is 0.1 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoperoxidase of monoclonal antibody to DGCR8 on formalin-fixed paraffin-embedded human colon. [antibody concentration 6 ug/ml]
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
- Protocol
- Protocol
