H00054487-D01P

antibody from Abnova Corporation

Targeting: DGCR8 C22orf12, DGCRK6, Gy1, pasha

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

H00054487-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00054487-D01P, RRID:AB_1673360

Product name

DGCR8 purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human DGCR8 protein.

Antigen sequence

METDESPSPLPCGPAGEAVMESRARPFQALPREQS
PPPPLQTSSGAEVMDVGSGGDGQSELPAEDPFNFY
GASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAV
RKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGA
ERDVRAECGLLLSPVSGDVHACPFGGSVGDGVGIG
GESADKKDEENELDQEKRVEYAVLDELEDFTDNLE
LDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDN
DVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETS
VQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSG
VPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSI
PCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRS
AELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGAL
GQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTV
KKFRTWAERRQFNREMKRKQAESERPILPANQKLI
TLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLK
VRPVYNFFECENPSEPFGASVTIDGVTYGSGTASS
KKLAKNKAARATLEILIPDFVKQTSEEKPKDSEEL
EYFNHISIEDSRVYELTSKAGLLSPYQILHECLKR
NHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGW
CKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGR
ESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKL
QEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCT
VDV


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

References

Submitted references

Identification of hepatic microvascular adhesion-related genes of human colon cancer cells using random homozygous gene perturbation.

Márquez J, Kohli M, Arteta B, Chang S, Li WB, Goldblatt M, Vidal-Vanaclocha F
International journal of cancer 2013 Nov;133(9):2113-22

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of DGCR8 expression in transfected 293T cell line (H00054487-T02) by DGCR8 MaxPab polyclonal antibody.Lane 1: DGCR8 transfected lysate(86.00 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

DGCR8 MaxPab rabbit polyclonal antibody. Western Blot analysis of DGCR8 expression in Jurkat.

Immunocytochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunofluorescence of purified MaxPab antibody to DGCR8 on HeLa cell. [antibody concentration 30 ug/ml]

Validation comment

Immunofluorescence

Protocol

Protocol