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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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- Product number
- H00054487-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00054487-D01, RRID:AB_10903100
- Product name
- DGCR8 MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human DGCR8 protein.
- Antigen sequence
METDESPSPLPCGPAGEAVMESRARPFQALPREQS
PPPPLQTSSGAEVMDVGSGGDGQSELPAEDPFNFY
GASLLSKGSFSKGRLLIDPNCSGHSPRTARHAPAV
RKFSPDLKLLKDVKISVSFTESCRSKDRKVLYTGA
ERDVRAECGLLLSPVSGDVHACPFGGSVGDGVGIG
GESADKKDEENELDQEKRVEYAVLDELEDFTDNLE
LDEEGAGGFTAKAIVQRDRVDEEALNFPYEDDFDN
DVDALLEEGLCAPKKRRTEEKYGGDSDHPSDGETS
VQPMMTKIKTVLKSRGRPPTEPLPDGWIMTFHNSG
VPVYLHRESRVVTWSRPYFLGTGSIRKHDPPLSSI
PCLHYKKMKDNEEREQSSDLTPSGDVSPVKPLSRS
AELEFPLDEPDSMGADPGPPDEKDPLGAEAAPGAL
GQVKAKVEVCKDESVDLEEFRSYLEKRFDFEQVTV
KKFRTWAERRQFNREMKRKQAESERPILPANQKLI
TLSVQDAPTKKEFVINPNGKSEVCILHEYMQRVLK
VRPVYNFFECENPSEPFGASVTIDGVTYGSGTASS
KKLAKNKAARATLEILIPDFVKQTSEEKPKDSEEL
EYFNHISIEDSRVYELTSKAGLLSPYQILHECLKR
NHGMGDTSIKFEVVPGKNQKSEYVMACGKHTVRGW
CKNKRVGKQLASQKILQLLHPHVKNWGSLLRMYGR
ESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKL
QEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCT
VDV- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of DGCR8 expression in transfected 293T cell line (H00054487-T02) by DGCR8 MaxPab polyclonal antibody.Lane 1: DGCR8 transfected lysate(86 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- DGCR8 MaxPab rabbit polyclonal antibody. Western Blot analysis of DGCR8 expression in Jurkat.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to DGCR8 on HeLa cell. [antibody concentration 30 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of DGCR8 transfected lysate using anti-DGCR8 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with DGCR8 purified MaxPab mouse polyclonal antibody (B01P) (H00054487-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
