Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
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Validation data
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- Product number
- AF2975 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse TFPI Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects mouse TFPI in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 20% cross-reactivity with recombinant human TFPI and less than 5% cross-reactivity with recombinant mouse TFPI-2 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
O54819
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of TFPI by Western Blot. Western blot shows lysate of mouse placenta tissue. PVDF membrane was probed with 2 µg/mL of Goat Anti-Mouse TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2975) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for TFPI at approximately 37-42 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Mouse TFPI by Simple WesternTM. Simple Western lane view shows lysates of mouse placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for TFPI at approximately 50-60 kDa (as indicated) using 100 µg/mL of Goat Anti-Mouse TFPI Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2975) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.