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Western blot
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Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- ELISA [1]
- Immunocytochemistry [1]
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- Product number
- MA5-51846 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Protein C Recombinant Mouse Monoclonal Antibody (HPC-4)
- Antibody type
- Monoclonal
- Antigen
- Other
- Description
- Specifically binds to a specific twelve peptide sequence (EDQVDPRLIDGK) in the activation region of the Protein C (spanning the thrombin cleavage site). Does not react with activated protein C. Has been shown to be cross-reactive with baboon Protein C but not with bovine or mouse Protein C.
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- HPC-4
- Vial size
- 200 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- ELISA analysis of Protein C using Protein C Recombinant Mouse Monoclocal Antibody (Product # MA5-51846; green line) and isotype control (Product # MA5-47750, anti-fluorescein; orange line). ELISA plate was coated with Multi Tag protein 2 at a concentration of 5 µg/mL and the primary antibody was titrated on a 3-fold serial dilution starting at 10,000 ng/mL. Anti-mouse IgG was used as secondary antibody for detection at a dilution of 1:4,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry analysis of Protein C in paraformaldehyde fixed HEK293 cells transfected with Protein C expressing plasmid (A) and non-transfected HEK293 cells (B), permeabilized with 0.15% Triton using Protein C Recombinant Mouse Monoclocal Antibody (Product # MA5-51846) at a 1:200 dilution for 1h followed by Alexa Fluor 488 secondary antibody (1:1,000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue).
