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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [3]
- Flow cytometry [1]
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- Product number
- ARG55148 - Provider product page
- Provider
- Arigo
- Product name
- anti-alpha-1D Adrenergic receptor antibody
- Antibody type
- Polyclonal
- Antigen
- KLH-conjugated synthetic peptide around aa. 1-30 (N-terminus) of Human alpha-1D Adrenergic receptor.
- Description
- Purification with Protein A and immunogen peptide.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 200 µl
- Storage
- For continuous use, store undiluted antibody at 2-8°C for up to 1-2 weeks. For long-term storage, aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Western blot: 35 µg of A549, LNCaP, PC-3 cell line and rat brain tissue lysate (from left to right) stained with ARG55148 anti-alpha-1D Adrenergic receptor antibody at 1:1000 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded H. kidney section stained with ARG55148 anti-alpha-1D Adrenergic receptor antibody at 1:100 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded H. small intestine section stained with ARG55148 anti-alpha-1D Adrenergic receptor antibody at 1:100 dilution.
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Immunohistochemistry: paraffin-embedded R. kidney section stained with ARG55148 anti-alpha-1D Adrenergic receptor antibody at 1:100 dilution.
Supportive validation
- Submitted by
- Arigo (provider)
- Main image
- Experimental details
- Flow Cytometry: MCF-7 cells stained with ARG55148 anti-alpha-1D Adrenergic receptor antibody (green) at 1:25 dilution or isotype control antibody (blue), followed by incubation with Alexa Fluor® 488 labelled secondary antibody.
