Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Flow cytometry [1]
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Validation data
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- Product number
- PA5-72171 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- alpha-1D Adrenoceptor Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ADRA1D in A549, LNCaP, PC-3 cell line and rat brain tissue lysate using 35 µg of protein per lane. Samples were probed with ADRA1D polyclonal antibody (Product # PA5-72171) with a dilution of 1:1000, and a goat anti-rabbit IgG H&L (HRP) secondary antibody with a dilution of 1:5000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of ADRA1D in MCF-7 cells (green) compared to an isotype control of rabbit IgG (blue). Samples were incubated with ADRA1D polyclonal antibody (Product # PA5-72171) using a dilution of 1:25 followed by a Alexa Fluor 488 goat anti-rabbit IgG secondary antibody with a dilution of 1:400.