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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29646 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- SERPINC1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HCT116. Predicted reactivity: Human (92%), Mouse (87%), Rat (85%), Pig (86%), Sheep (85%), Rhesus Monkey (90%), Bovine (83%), Guinea pig (85%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Proteomics and multivariate modelling reveal sex-specific alterations in distinct regions of human carotid atheroma.
Ward LJ, Olausson P, Li W, Yuan XM
Biology of sex differences 2018 Dec 29;9(1):54
Biology of sex differences 2018 Dec 29;9(1):54
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Antithrombin III using 30 µg of HCT116 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with an Antithrombin III polyclonal antibody (Product # PA5-29646) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of SERPINC1 was performed by separating 30 µg of human plasma by 12% SDS-PAGE. Proteins were transferred to a membrane and probed with a SERPINC1 Polyclonal Antibody (Product # PA5-29646) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Antithrombin III in methanol-fixed HeLa cells using an Antithrombin III polyclonal antibody (Product # PA5-29646) at a 1:500 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- SERPINC1 Polyclonal Antibody detects Antithrombin III protein at cytoplasm and nucleus on human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. SERPINC1 Polyclonal Antibody (Product # PA5-29646) diluted at 1:250. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
