Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Immunocytochemistry [1]
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Validation data
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- Product number
- H00022848-M02 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00022848-M02, RRID:AB_2219619
- Product name
- Mouse Monoclonal AAK1 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. AAK1 - AP2 associated kinase 1 (4B10)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: AAK1 Antibody (4B10) [H00022848-M02] - Analysis of AAK1 expression in transfected 293T cell line by AAK1 monoclonal antibody (M02), clone 4B10.Lane 1: AAK1 transfected lysate(52 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: AAK1 Antibody (4B10) [H00022848-M02] - Detection limit for recombinant GST tagged AAK1 is 0.1 ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: AAK1 Antibody (4B10) [H00022848-M02] - Analysis of monoclonal antibody to AAK1 on HeLa cell . Antibody concentration 10 ug/ml.