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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- R20316 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- Recombinant CD11b Antibody / MAC-1 (C-Terminal)
- Antibody type
- Monoclonal
- Description
- Protein A purified from animal origin-free supernatant
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Antibody clone number
- RM290
- Vial size
- 100 µl
- Storage
- Store the recombinant CD11b antibody at -20°C.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of lysate from human HL60 cells, nontreated (-) or treated (+) with DMSO, with recombinant CD11b antibody at 1:400 dilution. Expected molecular weight: 127~170 kDa depending on glycosylation level.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC testing of formalin fixed and paraffin embedded human tonsil tissue with recombinant CD11b antibody at 1:200 dilution. HIER: steam section in pH6 citrate buffer for 20 min and allow to cool prior to staining.
