- PA5-79532 - Invitrogen Antibodies ITGAM antibody

Fathy SM, El-Dash HA, Said NI
BMC complementary medicine and therapies 2021 Apr 26;21(1):130

Kalashnikova I, Chung SJ, Nafiujjaman M, Hill ML, Siziba ME, Contag CH, Kim T
Theranostics 2020;10(26):11863-11880

Peng D, Li J, Deng Y, Zhu X, Zhao L, Zhang Y, Li Z, Ou S, Li S, Jiang Y
Journal of neuroinflammation 2020 Nov 17;17(1):343

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Flow Cytometry of CD11b in HL-60 cells (blue line), isotype control rabbit IgG (green line) and unlabeled (red line). Samples were blocked with 10% goat serum, incubated with CD11b Polyclonal Antibody (Product # PA5-79532) at a dilution of 1 μg (per 1x10^6 cells), followed by DyLight®488 conjugated goat anti-rabbit IgG (for 30 minutes at 20°C) using 5-10 μg (per 1x10^6 cells) dilution.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Figure 7 Macrophage polarization in tissue sections harvested from PBS and A-nanoceria treated groups of CIA mice by immunohistology. (A) Images of tissue sections from animals treated with PBS or A-nanoceria. Sections of inflamed tissues (DAPI in blue) displayed a high concentration of infiltrating macrophages into the paws (CD11b in red). The number of M1 macrophages (iNOS in pink) decreased upon treatment with A-nanoceria. Tissues from animals treated with A-nanoceria revealed a high number of M2 macrophages (Arg-1 in yellow). Immunohistology of tissue sections harvested from normal mice can be found in Figure S6 ; (B) Summarized plot of CD11b, iNOS and Arg-1 MFI signals in ROI from images. In each group, we took 5 fluorescence images from two mice tissues and plotted 5 random ROIs for each image (N = 25. error bar = SD, p -value: *** p < 0.0005).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 4 PAS-Na mitigates Mn-induced BV2 cells activation and microglia proliferation in basal ganglia of rats by inhibited NF-kappaB pathway activation. a , b The rats were treated with 5, 10, and 20 mg/kg MnCl 2 for 8 weeks. a The protein expressions of CD11b in the basal ganglia of rats was analyzed by western blot ( n = 4 per group). b The protein expressions of p-p65, p65, p-IkappaB-alpha, and IkappaB-alpha in the basal ganglia of rats was detected by western blot ( n = 4 per group). c - d The rats were treated with 20 mg/kg MnCl 2 for 8 weeks and then treated with 100, 200, and 300 mg/kg PAS-Na for an additional 6 weeks. c The protein expression of CD11b in the basal ganglia of rats were analyzed by western blot ( n = 4 per group). d Immunohistochemical results of CD11b in the basal ganglia of rats ( n = 3 per group). Scale bars: 100 mum. e The protein expressions of p-p65, p65, p-IkappaB-alpha, and IkappaB-alpha in the basal ganglia of rats were detected by western blot ( n = 4 per group). The protein expression was normalized by beta-actin or corresponding total protein content. Data are presented as mean +- SD. * p < 0.05 and ** p < 0.01: significant as compared to the control group; # p < 0.05 and ## p < 0.01: significant as compared to corresponding Mn-treated group

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
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Experimental details: Fig. 7 Representative cropped blots with relative expression levels of the striatal CD11b, TGF-beta, and GDNF in different animal groups. The full length blots are presented in supplementary figure 3 . Relative expression of CD11b ( a ), GDNF ( b ), and TGF-beta ( c ) in striatum. C = control group; PQ = Paraquat (alone)-induced group; PQ+PSE = PQ-induced group treated with PSE; PQ+PJ = PQ-induced group treated with PJ. Data are expressed as mean +- S.D. of 10 mice in each group. **: P < 0.01, ***: P < 0.001, and ****: P < 0.0001 compared with control group; ####: P < 0.0001 compared with PQ group

PA5-79532