14-0118-37

antibody from Invitrogen Antibodies
Targeting: ITGAM CD11B, CR3A, MAC-1
Provider product page for 14-0118-37
Immunohistochemistry
Flow cytometry
Blocking/Neutralizing
Other assay

Antibody data

Product number
14-0118-37 - Provider product page
Provider
Invitrogen Antibodies
Product name
CD11b Monoclonal Antibody (ICRF44), eBioscience™
Antibody type
Monoclonal
Antigen
Other
Description
Description: The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions. Applications Reported: The ICRF44 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining on formaldehyde fixed tissue. ICRF44 has also been reported in inhibition of aggregation of PMNs. Applications Tested: The ICRF44 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Purity: Greater than 90%, as determined by SDS-PAGE. Aggregation: Less than 10%, as determined by HPLC. Filtration: 0.2 µm post-manufacturing filtered.
Reactivity
Human
Host
Mouse
Isotype
IgG
Antibody clone number
ICRF44
Vial size
2 mg
Concentration
0.5 mg/mL
Storage
4° C
ITGAM protein structure - 14-0118-37 shown in red.