101203

antibody from BioLegend

Targeting: ITGAM CD11B, CR3A, MAC-1

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

101203

Provider

BioLegend

Proper citation

BioLegend Cat#101203, RRID:AB_312786

Product name

Biotin anti-mouse/human CD11b

Antibody type

Monoclonal

Antigen

C57BL/10 splenocytes

Description

CD11b is a 170 kD glycoprotein also known as αM integrin, Mac-1 α subunit, Mol, CR3, and Ly-40. CD11b is a member of the integrin family, primarily expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b non-covalently associates with CD18 (β2 integrin) to form Mac-1. Mac-1 plays an important role in cell-cell interaction by binding its ligands ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), iC3b, and fibrinogen.

Reactivity

Human, Mouse, Rabbit, Simian

Host

Rat

Conjugate

Biotin

Isotype

IgG

Vial size

50 µg

Concentration

0.5 mg/ml

Storage

The CD11b antibody solution should be stored undiluted at 4°C. Do not freeze.

References

Submitted references

Basophils and the T helper 2 environment can promote the development of lupus nephritis.

Charles N, Hardwick D, Daugas E, Illei GG, Rivera J
Nature medicine 2010 Jun;16(6):701-7

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Peptide-MHC class II complex stability governs CD4 T cell clonal selection.

Baumgartner CK, Ferrante A, Nagaoka M, Gorski J, Malherbe LP
Journal of immunology (Baltimore, Md. : 1950) 2010 Jan 15;184(2):573-81

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Tumor-infiltrating regulatory dendritic cells inhibit CD8+ T cell function via L-arginine metabolism.

Norian LA, Rodriguez PC, O'Mara LA, Zabaleta J, Ochoa AC, Cella M, Allen PM
Cancer research 2009 Apr 1;69(7):3086-94

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Neutrophils transiently infiltrate intra-abdominal fat early in the course of high-fat feeding.

Elgazar-Carmon V, Rudich A, Hadad N, Levy R
Journal of lipid research 2008 Sep;49(9):1894-903

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Deficiency of decay-accelerating factor and complement receptor 1-related gene/protein y on murine platelets leads to complement-dependent clearance by the macrophage phagocytic receptor CRIg.

Kim DD, Miwa T, Kimura Y, Schwendener RA, van Lookeren Campagne M, Song WC
Blood 2008 Aug 15;112(4):1109-19

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Core binding factors are necessary for natural killer cell development and cooperate with Notch signaling during T-cell specification.

Guo Y, Maillard I, Chakraborti S, Rothenberg EV, Speck NA
Blood 2008 Aug 1;112(3):480-92

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Resistance to experimental autoimmune encephalomyelitis and impaired IL-17 production in protein kinase C theta-deficient mice.

Tan SL, Zhao J, Bi C, Chen XC, Hepburn DL, Wang J, Sedgwick JD, Chintalacharuvu SR, Na S
Journal of immunology (Baltimore, Md. : 1950) 2006 Mar 1;176(5):2872-9

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Mac-1+ cells are the predominant subset in the early hepatic lesions of mice infected with Francisella tularensis.

Rasmussen JW, Cello J, Gil H, Forestal CA, Furie MB, Thanassi DG, Benach JL
Infection and immunity 2006 Dec;74(12):6590-8

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

C57BL/6 mouse bone marrow cells stained with biotinylated M1/70, followed by Sav-PE

Conjugate

Biotin