301312

antibody from BioLegend

Targeting: ITGAM CD11B, CR3A, MAC-1

Immunocytochemistry (Recommended by provider)

Immunohistochemistry (Recommended by provider)

Flow cytometry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Recommended by provider)

Antibody Data

Product number

301312

Provider

BioLegend

Proper citation

BioLegend Cat#301312, RRID:AB_314164

Product name

LEAF™ Purified anti-human CD11b [ICRF44]

Antibody type

Monoclonal

Antigen

CD11b

Description

CD11b is a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages, dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils. It is also involved in granulocyte adhesion, phagocytosis, and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen, and Factor X.

Reactivity

Human, Porcine, Simian

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Vial size

500 µg

Concentration

1 mg/ml

Storage

The antibody solution should be stored undiluted at 4 °C. This LEAF™ solution contains no preservative; handle under aseptic conditions.

References

Submitted references

Gelatinase contributes to the pathogenesis of endocarditis caused by Enterococcus faecalis.

Thurlow LR, Thomas VC, Narayanan S, Olson S, Fleming SD, Hancock LE
Infection and immunity 2010 Nov;78(11):4936-43

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Basophils and the T helper 2 environment can promote the development of lupus nephritis.

Charles N, Hardwick D, Daugas E, Illei GG, Rivera J
Nature medicine 2010 Jun;16(6):701-7

Flow cytometry

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Human peripheral blood granulocytes stained with LEAF™ purified ICRF44, followed by anti-mouse IgGs FITC

Conjugate

Unconjugated