Antibody data
- Antibody Data
- Antigen structure
- References [6]
- Comments [0]
- Validations
- Flow cytometry [1]
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- Product number
- 301402 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#301402, RRID:AB_314166
- Product name
- Purified anti-human CD11b (activated)
- Antibody type
- Monoclonal
- Antigen
- CD11b (activated)
- Description
- The CBRM1/5 antibody reacts with an activated form of human CD11b, a 165-170 kD type I transmembrane glycoprotein also known as αM integrin, Mac-1, CR3, and C3biR. CD11b non-covalently associates with integrin β2 (CD18) and is expressed on granulocytes, monocytes/macrophages dendritic cells, NK cells, and subsets of T and B cells. CD11b/CD18 is critical for the transendothelial migration of monocytes and neutrophils, as well as adhesion, phagocytosis and neutrophil activation. CD11b/CD18 interacts with ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4, CD14, CD23, heparin, iC3b, fibrinogen and Factor X. The CBRM1/5 antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol-esters. This antibody does not recognize "non-activated" CD11b. The epitope recognized by CBRM1/5 is contained in the I domain of the α chain. The CBRM1/5 antibody blocks cell adhesion to fibrinogen and ICAM-1.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- The antibody solution should be stored undiluted at 4° C.
Submitted references Hax1 regulates neutrophil adhesion and motility through RhoA.
Infection of dendritic cells with herpes simplex virus type 1 induces rapid degradation of CYTIP, thereby modulating adhesion and migration.
The Vi capsular polysaccharide prevents complement receptor 3-mediated clearance of Salmonella enterica serotype Typhi.
Human eosinophils express RAGE, produce RAGE ligands, exhibit PKC-delta phosphorylation and enhanced viability in response to the RAGE ligand, S100B.
Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia.
Dissection of the hyperadhesive phenotype of airway eosinophils in asthma.
Cavnar PJ, Berthier E, Beebe DJ, Huttenlocher A
The Journal of cell biology 2011 May 2;193(3):465-73
The Journal of cell biology 2011 May 2;193(3):465-73
Infection of dendritic cells with herpes simplex virus type 1 induces rapid degradation of CYTIP, thereby modulating adhesion and migration.
Theodoridis AA, Eich C, Figdor CG, Steinkasserer A
Blood 2011 Jul 7;118(1):107-15
Blood 2011 Jul 7;118(1):107-15
The Vi capsular polysaccharide prevents complement receptor 3-mediated clearance of Salmonella enterica serotype Typhi.
Wilson RP, Winter SE, Spees AM, Winter MG, Nishimori JH, Sanchez JF, Nuccio SP, Crawford RW, Tükel Ç, Bäumler AJ
Infection and immunity 2011 Feb;79(2):830-7
Infection and immunity 2011 Feb;79(2):830-7
Human eosinophils express RAGE, produce RAGE ligands, exhibit PKC-delta phosphorylation and enhanced viability in response to the RAGE ligand, S100B.
Curran CS, Bertics PJ
International immunology 2011 Dec;23(12):713-28
International immunology 2011 Dec;23(12):713-28
Functional heterogeneity and differential priming of circulating neutrophils in human experimental endotoxemia.
Pillay J, Ramakers BP, Kamp VM, Loi AL, Lam SW, Hietbrink F, Leenen LP, Tool AT, Pickkers P, Koenderman L
Journal of leukocyte biology 2010 Jul;88(1):211-20
Journal of leukocyte biology 2010 Jul;88(1):211-20
Dissection of the hyperadhesive phenotype of airway eosinophils in asthma.
Barthel SR, Jarjour NN, Mosher DF, Johansson MW
American journal of respiratory cell and molecular biology 2006 Sep;35(3):378-86
American journal of respiratory cell and molecular biology 2006 Sep;35(3):378-86
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Supportive validation
- Submitted by
- BioLegend (provider)
- Main image
- Experimental details
- PMA activated human granulocytes stained with CBRM1/5 FITC
- Conjugate
- Unconjugated