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Immunocytochemistry
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [3]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB1699 - Provider product page
- Provider
- R&D Systems
- Product name
- Human CD11b/Integrin alpha M Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human CD11b/Integrin alpha M.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- ICRF44
- Vial size
- 500 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Activation of the plasma kallikrein-kinin system on human lung epithelial cells.
Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells.
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Vergiliana JF, Asokananthan N, Stewart GA
Biological chemistry 2010 Sep;391(9):1067-77
Biological chemistry 2010 Sep;391(9):1067-77
Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells.
Allen CE, Li L, Peters TL, Leung HC, Yu A, Man TK, Gurusiddappa S, Phillips MT, Hicks MJ, Gaikwad A, Merad M, McClain KL
Journal of immunology (Baltimore, Md. : 1950) 2010 Apr 15;184(8):4557-67
Journal of immunology (Baltimore, Md. : 1950) 2010 Apr 15;184(8):4557-67
Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
Immunology 2004 Jun;112(2):274-89
Immunology 2004 Jun;112(2):274-89
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- CD11b/Integrin alpha M in Human PBMCs. CD11b/Integrin alpha M was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB1699) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of CD11b/Integrin alpha M in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB1699, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.
