sc-8014

antibody from Santa Cruz Biotechnology

Targeting: IKBKB IKK-beta, IKK2, IKKB, NFKBIKB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

sc-8014

Provider

Santa Cruz Biotechnology

Proper citation

Santa Cruz Biotechnology Cat#sc-8014, RRID:AB_627785

Product name

Anti-IKBKB

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Reactivity

Human

Host

Mouse

References

Submitted references

Cytosolic Hsp60 is involved in the NF-kappaB-dependent survival of cancer cells via IKK regulation.

Chun JN, Choi B, Lee KW, Lee DJ, Kang DH, Lee JY, Song IS, Kim HI, Lee SH, Kim HS, Lee NK, Lee SY, Lee KJ, Kim J, Kang SW
PloS one 2010 Mar 23;5(3):e9422

---

Nuclear factor kappaB transcription factors are coexpressed and convey a poor outcome in ovarian cancer.

Annunziata CM, Stavnes HT, Kleinberg L, Berner A, Hernandez LF, Birrer MJ, Steinberg SM, Davidson B, Kohn EC
Cancer 2010 Jul 1;116(13):3276-84

---

NEMO-binding domains of both IKKalpha and IKKbeta regulate IkappaB kinase complex assembly and classical NF-kappaB activation.

Solt LA, Madge LA, May MJ
The Journal of biological chemistry 2009 Oct 2;284(40):27596-608

---

The human cytomegalovirus virion possesses an activated casein kinase II that allows for the rapid phosphorylation of the inhibitor of NF-kappaB, IkappaBalpha.

Nogalski MT, Podduturi JP, DeMeritt IB, Milford LE, Yurochko AD
Journal of virology 2007 May;81(10):5305-14

---

Notch3 and pre-TCR interaction unveils distinct NF-kappaB pathways in T-cell development and leukemia.

Vacca A, Felli MP, Palermo R, Di Mario G, Calce A, Di Giovine M, Frati L, Gulino A, Screpanti I
The EMBO journal 2006 Mar 8;25(5):1000-8

---

The NEMO mutation creating the most-upstream premature stop codon is hypomorphic because of a reinitiation of translation.

Puel A, Reichenbach J, Bustamante J, Ku CL, Feinberg J, Döffinger R, Bonnet M, Filipe-Santos O, de Beaucoudrey L, Durandy A, Horneff G, Novelli F, Wahn V, Smahi A, Israel A, Niehues T, Casanova JL
American journal of human genetics 2006 Apr;78(4):691-701

---

Positive and negative regulation of EAAT2 by NF-kappaB: a role for N-myc in TNFalpha-controlled repression.

Sitcheran R, Gupta P, Fisher PB, Baldwin AS
The EMBO journal 2005 Feb 9;24(3):510-20

---

Activation of the NF-kappaB pathway in human cytomegalovirus-infected cells is necessary for efficient transactivation of the major immediate-early promoter.

DeMeritt IB, Milford LE, Yurochko AD
Journal of virology 2004 May;78(9):4498-507

---

PKCθ mediates pre-TCR signaling and contributes to Notch3-induced T-cell leukemia

Maria Pia Felli, Alessandra Vacca, Angelica Calce, Diana Bellavia, Antonio F Campese, Rita Grillo, Monica Di Giovine, Saula Checquolo, Claudio Talora, Rocco Palermo, Giuseppina Di Mario, Luigi Frati, Alberto Gulino, Isabella Screpanti
Oncogene 2004 Dec;24(6):992-1000

Western blot

Supportive validation

Submitted by

per

Main image

Experimental details

Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.

Validation comment

No bands detected.

Primary Ab dilution

1:500

Secondary Ab dilution

1:7000

Lane 1

Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6

Lane 2

RT-4

Lane 3

U-251MG sp

Lane 4

Human Plasma

Lane 5

Liver

Lane 6

Tonsil

Theoretical target weight

[kDa] 55

Immunohistochemistry

Supportive validation

Submitted by

per

Main image

Experimental details

Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.

Validation comment

Staining pattern partly consistent with experimental and/or bioinformatic data.