Antibody data
- Antibody Data
- Antigen structure
- References [9]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
Reference
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- Product number
- sc-8014 - Provider product page
- Provider
- Santa Cruz Biotechnology
- Proper citation
- Santa Cruz Biotechnology Cat#sc-8014, RRID:AB_627785
- Product name
- Anti-IKBKB
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Reactivity
- Human
- Host
- Mouse
Submitted references Cytosolic Hsp60 is involved in the NF-kappaB-dependent survival of cancer cells via IKK regulation.
Nuclear factor kappaB transcription factors are coexpressed and convey a poor outcome in ovarian cancer.
NEMO-binding domains of both IKKalpha and IKKbeta regulate IkappaB kinase complex assembly and classical NF-kappaB activation.
The human cytomegalovirus virion possesses an activated casein kinase II that allows for the rapid phosphorylation of the inhibitor of NF-kappaB, IkappaBalpha.
Notch3 and pre-TCR interaction unveils distinct NF-kappaB pathways in T-cell development and leukemia.
The NEMO mutation creating the most-upstream premature stop codon is hypomorphic because of a reinitiation of translation.
Positive and negative regulation of EAAT2 by NF-kappaB: a role for N-myc in TNFalpha-controlled repression.
Activation of the NF-kappaB pathway in human cytomegalovirus-infected cells is necessary for efficient transactivation of the major immediate-early promoter.
PKCθ mediates pre-TCR signaling and contributes to Notch3-induced T-cell leukemia
Chun JN, Choi B, Lee KW, Lee DJ, Kang DH, Lee JY, Song IS, Kim HI, Lee SH, Kim HS, Lee NK, Lee SY, Lee KJ, Kim J, Kang SW
PloS one 2010 Mar 23;5(3):e9422
PloS one 2010 Mar 23;5(3):e9422
Nuclear factor kappaB transcription factors are coexpressed and convey a poor outcome in ovarian cancer.
Annunziata CM, Stavnes HT, Kleinberg L, Berner A, Hernandez LF, Birrer MJ, Steinberg SM, Davidson B, Kohn EC
Cancer 2010 Jul 1;116(13):3276-84
Cancer 2010 Jul 1;116(13):3276-84
NEMO-binding domains of both IKKalpha and IKKbeta regulate IkappaB kinase complex assembly and classical NF-kappaB activation.
Solt LA, Madge LA, May MJ
The Journal of biological chemistry 2009 Oct 2;284(40):27596-608
The Journal of biological chemistry 2009 Oct 2;284(40):27596-608
The human cytomegalovirus virion possesses an activated casein kinase II that allows for the rapid phosphorylation of the inhibitor of NF-kappaB, IkappaBalpha.
Nogalski MT, Podduturi JP, DeMeritt IB, Milford LE, Yurochko AD
Journal of virology 2007 May;81(10):5305-14
Journal of virology 2007 May;81(10):5305-14
Notch3 and pre-TCR interaction unveils distinct NF-kappaB pathways in T-cell development and leukemia.
Vacca A, Felli MP, Palermo R, Di Mario G, Calce A, Di Giovine M, Frati L, Gulino A, Screpanti I
The EMBO journal 2006 Mar 8;25(5):1000-8
The EMBO journal 2006 Mar 8;25(5):1000-8
The NEMO mutation creating the most-upstream premature stop codon is hypomorphic because of a reinitiation of translation.
Puel A, Reichenbach J, Bustamante J, Ku CL, Feinberg J, Döffinger R, Bonnet M, Filipe-Santos O, de Beaucoudrey L, Durandy A, Horneff G, Novelli F, Wahn V, Smahi A, Israel A, Niehues T, Casanova JL
American journal of human genetics 2006 Apr;78(4):691-701
American journal of human genetics 2006 Apr;78(4):691-701
Positive and negative regulation of EAAT2 by NF-kappaB: a role for N-myc in TNFalpha-controlled repression.
Sitcheran R, Gupta P, Fisher PB, Baldwin AS
The EMBO journal 2005 Feb 9;24(3):510-20
The EMBO journal 2005 Feb 9;24(3):510-20
Activation of the NF-kappaB pathway in human cytomegalovirus-infected cells is necessary for efficient transactivation of the major immediate-early promoter.
DeMeritt IB, Milford LE, Yurochko AD
Journal of virology 2004 May;78(9):4498-507
Journal of virology 2004 May;78(9):4498-507
PKCθ mediates pre-TCR signaling and contributes to Notch3-induced T-cell leukemia
Maria Pia Felli, Alessandra Vacca, Angelica Calce, Diana Bellavia, Antonio F Campese, Rita Grillo, Monica Di Giovine, Saula Checquolo, Claudio Talora, Rocco Palermo, Giuseppina Di Mario, Luigi Frati, Alberto Gulino, Isabella Screpanti
Oncogene 2004 Dec;24(6):992-1000
Oncogene 2004 Dec;24(6):992-1000
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Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Western blot analysis of antibody specificity using a routine panel composed of IgG/HSA-depleted human plasma and protein lysates from selected human tissues and cell lines.
- Validation comment
- No bands detected.
- Primary Ab dilution
- 1:500
- Secondary Ab dilution
- 1:7000
- Lane 1
- Marker [kDa]: 230, 110, 82, 49.3, 32.2, 25.5, 17.6
- Lane 2
- RT-4
- Lane 3
- U-251MG sp
- Lane 4
- Human Plasma
- Lane 5
- Liver
- Lane 6
- Tonsil
- Theoretical target weight
- [kDa] 55
Supportive validation
- Submitted by
- per
- Main image
- Experimental details
- Immunohistochemical staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.
- Validation comment
- Staining pattern partly consistent with experimental and/or bioinformatic data.