Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-33214 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Mouse Monoclonal IKK beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. An approx. 87 kDa band should be observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Simple Western: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214] - Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system. *Non-specific interaction with the 230 kDa standard may be seen with this antibody.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214] - Analysis in Daudi cell lysate using IKK beta antibody at 5 ug/ml.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214] - Antibodoy was used to immunoprecipitate IKKb from 1x10^6 Daudi cells and the immuno-precipitated protein was detected by western blotting using NB100-56509. IKKb was detected as a protein of ~87 kD.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: IKK beta Antibody (10A9B6) - Azide Free [NBP2-33214] - Analysis using the Alexa Fluor (R) 488 conjugate. Staining of IKK in HEK 293 cells using 0.1 ug of this antibody. Green histogram represents the isotype control , red represents the IKK antibody.