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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
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- Product number
- MAB7155 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rat Monoclonal IKK beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects mouse IKK beta in direct ELISAs and Western blots.
- Reactivity
- Mouse
- Host
- Rat
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Uncaria tomentosa improves insulin sensitivity and inflammation in experimental NAFLD.
IL6-mediated inflammatory loop reprograms normal to epithelial-mesenchymal transition+ metastatic cancer stem cells in preneoplastic liver of transforming growth factor beta-deficient β2-spectrin+/- mice.
Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation.
Araujo LCC, Feitosa KB, Murata GM, Furigo IC, Teixeira SA, Lucena CF, Ribeiro LM, Muscará MN, Costa SKP, Donato J Jr, Bordin S, Curi R, Carvalho CRO
Scientific reports 2018 Jul 20;8(1):11013
Scientific reports 2018 Jul 20;8(1):11013
IL6-mediated inflammatory loop reprograms normal to epithelial-mesenchymal transition+ metastatic cancer stem cells in preneoplastic liver of transforming growth factor beta-deficient β2-spectrin+/- mice.
Mitra A, Yan J, Xia X, Zhou S, Chen J, Mishra L, Li S
Hepatology (Baltimore, Md.) 2017 Apr;65(4):1222-1236
Hepatology (Baltimore, Md.) 2017 Apr;65(4):1222-1236
Phosphorylation and linear ubiquitin direct A20 inhibition of inflammation.
Wertz IE, Newton K, Seshasayee D, Kusam S, Lam C, Zhang J, Popovych N, Helgason E, Schoeffler A, Jeet S, Ramamoorthi N, Kategaya L, Newman RJ, Horikawa K, Dugger D, Sandoval W, Mukund S, Zindal A, Martin F, Quan C, Tom J, Fairbrother WJ, Townsend M, Warming S, DeVoss J, Liu J, Dueber E, Caplazi P, Lee WP, Goodnow CC, Balazs M, Yu K, Kolumam G, Dixit VM
Nature 2015 Dec 17;528(7582):370-5
Nature 2015 Dec 17;528(7582):370-5
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Detection of Mouse IKK beta by Western Blot. Western blot shows lysates of BaF3 mouse pro-B cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse IKK beta Monoclonal Antibody (Catalog # MAB7155) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody. A specific band was detected for IKK beta at approximately 87 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
