PA5-36718

antibody from Invitrogen Antibodies

Targeting: IKBKB IKK-beta, IKK2, IKKB, NFKBIKB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-36718

Provider

Invitrogen Antibodies

Product name

Phospho-IKK alpha/beta (Ser180, Ser181) Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody detects endogenous protein at a molecular weight of 85 kDa (alpha) and 87 kDa (beta).

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-IKK alpha/beta (Ser180, Ser181) in Lane 1: A549 cell lysate treated with lps (100 ng/mL,15 mins), Lane 2: Raw264.7 cell lysate treated with lps (100 ng/mL,15 mins), Lane 3: H9C2 cell lysate treated with lps (100 ng/mL,15 mins). Samples were incubated with Phospho-IKK alpha/beta (Ser180, Ser181) polyclonal antibody (Product # PA5-36718) at a dilution of 1:500.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Phospho-IKK alpha pSer180+IKK beta pSer181 using Phospho-IKK alpha pSer180+IKK beta pSer181 polyclonal antibody (Product # PA5-36718) at a dilution of 1:500. Lane 1: A549 cell lysate treated with LPS (100 ng/mL, 15 min), Lane 2: Raw264.7 cell lysate treated with LPS (100 ng/mL, 15 min), Lane 3: H9C2 cell lysate treated with LPS (100 ng/mL, 15 min).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of Phospho-IKK alpha pSer180+IKK beta pSer181 in paraffin-embedded human colorectal carcinoma using Phospho-IKK alpha pSer180+IKK beta pSer181 polyclonal antibody (Product # PA5-36718) at a dilution of 1:50.