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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- ELISA [1]
- Proximity ligation assay [1]
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- Product number
- H00003551-M04 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00003551-M04, RRID:AB_622335
- Product name
- IKBKB monoclonal antibody (M04), clone 1A3
- Antibody type
- Monoclonal
- Description
- Mouse monoclonal antibody raised against a partial recombinant IKBKB.
- Antigen sequence
WSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQET
GEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPN
VVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKY
LNQFENCCGLREG- Isotype
- IgG
- Antibody clone number
- 1A3
- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- IKBKB monoclonal antibody (M04), clone 1A3. Western Blot analysis of IKBKB expression in IMR-32.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Detection limit for recombinant GST tagged IKBKB is 0.3 ng/ml as a capture antibody.
- Validation comment
- Sandwich ELISA (Recombinant protein)
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Proximity Ligation Analysis of protein-protein interactions between BIRC3 and IKBKB. HeLa cells were stained with anti-BIRC3 rabbit purified polyclonal 1:1200 and anti-IKBKB mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).
- Validation comment
- In situ Proximity Ligation Assay (Cell)
