H00003551-M09

antibody from Abnova Corporation

Targeting: IKBKB IKK-beta, IKK2, IKKB, NFKBIKB

ELISA (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003551-M09

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003551-M09, RRID:AB_733194

Product name

IKBKB monoclonal antibody (M09), clone 2C3

Antibody type

Monoclonal

Description

Mouse monoclonal antibody raised against a partial recombinant IKBKB.

Antigen sequence

WSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQET
GEQIAIKQCRQELSPRNRERWCLEIQIMRRLTHPN
VVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLRKY
LNQFENCCGLREG


Isotype

IgG

Antibody clone number

2C3

Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

ELISA

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Detection limit for recombinant GST tagged IKBKB is approximately 0.1ng/ml as a capture antibody.

Validation comment

Sandwich ELISA (Recombinant protein)

Protocol

Protocol

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between TNFRSF1A and IKBKB. HeLa cells were stained with anti-TNFRSF1A rabbit purified polyclonal 1:1200 and anti-IKBKB mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)