H00003551-D01P

antibody from Abnova Corporation

Targeting: IKBKB IKK-beta, IKK2, IKKB, NFKBIKB

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00003551-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00003551-D01P, RRID:AB_1677501

Product name

IKBKB purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human IKBKB protein.

Antigen sequence

MSWSPSLTTQTCGAWEMKERLGTGGFGNVIRWHNQ
ETGEQIAIKQCRQELSPRNRERWCLEIQIMRRLTH
PNVVAARDVPEGMQNLAPNDLPLLAMEYCQGGDLR
KYLNQFENCCGLREGAILTLLSDIASALRYLHENR
IIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQ
GSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGT
LAFECITGFRPFLPNWQPVQCVRMWPGTVAHSCNP
STLGGRGRWIS


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of IKBKB expression in transfected 293T cell line (H00003551-T01) by IKBKB MaxPab polyclonal antibody.Lane 1: IKBKB transfected lysate(29.20 KDa).Lane 2: Non-transfected lysate.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between IKBKB and CHUK. HeLa cells were stained with anti-IKBKB rabbit purified polyclonal 1:1200 and anti-CHUK mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)