PAB10247

antibody from Abnova Corporation

Targeting: IKBKB IKK-beta, IKK2, IKKB, NFKBIKB

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PAB10247

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#PAB10247, RRID:AB_1676601

Product name

IKBKB polyclonal antibody

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against synthetic peptide of IKBKB.

Storage

Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunoblot of IKBKB polyclonal antibody (Cat # PAB10247) reacted with HeLa cell extract.All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.The membrane was blocked in 5% dry milk for 2 h.After washing, a 1:500 dilution of the primary antibody was added to the membraneand incubated for 2 h.Washes with buffer were performed 4 times for 5' each.The immunoblot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h.Washes with TBS preceded color development.

Immunohistochemistry

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Immunohistochemical staining with IKBKB polyclonal antibody (Cat # PAB10247) was diluted 1 : 500 to detect IKBKB in human placenta tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Validation comment

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)