Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB10247 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10247, RRID:AB_1676601
- Product name
- IKBKB polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of IKBKB.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoblot of IKBKB polyclonal antibody (Cat # PAB10247) reacted with HeLa cell extract.All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature.The membrane was blocked in 5% dry milk for 2 h.After washing, a 1:500 dilution of the primary antibody was added to the membraneand incubated for 2 h.Washes with buffer were performed 4 times for 5' each.The immunoblot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h.Washes with TBS preceded color development.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunohistochemical staining with IKBKB polyclonal antibody (Cat # PAB10247) was diluted 1 : 500 to detect IKBKB in human placenta tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)