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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
- Other assay [2]
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- Product number
- PA5-85246 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IKK beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Keep as concentrated solution. Predicted reactivity: Mouse (80%). Positive Control: Jurkat, Raji, K562, THP-1, NCI-H929, DDDDK-tagged IKBKB-transfected 293T. Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.96 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IKK beta Polyclonal Antibody detects IKK beta protein at cytoplasm by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: IKK beta protein stained by IKK beta Polyclonal Antibody (Product # PA5-85246) diluted at 1:500. Blue: Hoechst 33342 staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation analysis of IKK beta in A431 whole cell extracts with IKK beta polyclonal antibody (Product # PA5-85246) using 5 µg of sample, followed by anti-Rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunoprecipitation of IKK beta protein from A431 whole cell extracts using 5 µg of IKK beta Polyclonal Antibody (Product # PA5-85246). Western blot analysis was performed using IKK beta Polyclonal Antibody (Product # PA5-85246) diluted at 1:500.
