Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [5]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21683 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IKK beta Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: A431, PC-12, DDDDK-human IKBKB-transfected 293T. Predicted reactivity: Mouse (95%), Rat (92%), Pig (92%), Chicken (82%), Rhesus Monkey (100%), Bovine (92%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.41 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IKK beta using 30 µg PC-12 whole cell lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an IKK beta polyclonal antibody (Product # PA5-21683) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IKK beta using 30 µg of A431 lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with an IKK beta polyclonal antibody (Product # PA5-21683) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IKK beta was performed by separating 30 µg of various whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a IKK beta Polyclonal Antibody (Product # PA5-21683) at a dilution of 1:500 and a HRP-conjugated anti-rabbit IgG secondary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IKK beta was performed by separating 30 µg of Whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a IKK beta Polyclonal Antibody (Product # PA5-21683) at a dilution of 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IKK beta Polyclonal Antibody detects IKK beta protein by western blot analysis. A. 30 µg 293T whole cell extract. B. 30 µg whole cell extract of DDDDK-human IKBKB-transfected 293T cells.7.5 % SDS-PAGE. IKK beta Polyclonal Antibody (Product # PA5-21683) dilution: 1:5,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IKK beta in paraformaldehyde-fixed A431 cells using an IKK beta polyclonal antibody (Product # PA5-21683) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A431, using IKK beta (Product # PA5-21683) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using IKK beta (Product # PA5-21683) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.