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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [59]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
- Flow cytometry [4]
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- Product number
- NB100-56509 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56509, RRID:AB_838417
- Product name
- Mouse Monoclonal IKK beta Antibody
- Antibody type
- Monoclonal
- Description
- Protein G purified. An approx. 87 kDa band should be observed.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1.0 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Mechanism of dysfunction of human variants of the IRAK4 kinase and a role for its kinase activity in interleukin-1 receptor signaling.
Uncovering inherent cellular plasticity of multiciliated ependyma leading to ventricular wall transformation and hydrocephalus.
NFκB signaling in alveolar rhabdomyosarcoma.
Spontaneous Development of Cutaneous Squamous Cell Carcinoma in Mice with Cell-specific Deletion of Inhibitor of κB Kinase 2.
Inflammation Improves Glucose Homeostasis through IKKβ-XBP1s Interaction.
HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains.
Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model.
The protein phosphatase 2A regulatory subunit B56γ mediates suppression of T cell receptor (TCR)-induced nuclear factor-κB (NF-κB) activity.
T cell receptor signals to NF-κB are transmitted by a cytosolic p62-Bcl10-Malt1-IKK signalosome.
Noncanonical NF-κB signaling is limited by classical NF-κB activity.
Sirtuin-1 (SIRT1) is required for promoting chondrogenic differentiation of mesenchymal stem cells.
Curcumin inhibits tumor growth and angiogenesis in an orthotopic mouse model of human pancreatic cancer.
TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2.
IKKβ overexpression leads to pathologic lesions in stratified epithelia and exocrine glands and to tumoral transformation of oral epithelia.
A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IκB kinase activation and sensitizes tumor cells to apoptosis by cytokines and chemotherapeutic agents.
Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway.
Targeted deletion of hepatocyte Ikkbeta confers growth advantages.
HTLV-1 Tax is a critical lipid raft modulator that hijacks IkappaB kinases to the microdomains for persistent activation of NF-kappaB.
cIAP1, cIAP2, and XIAP act cooperatively via nonredundant pathways to regulate genotoxic stress-induced nuclear factor-kappaB activation.
Inhibition of IkappaB kinase-nuclear factor-kappaB signaling pathway by 3,5-bis(2-flurobenzylidene)piperidin-4-one (EF24), a novel monoketone analog of curcumin.
Coronarin D, a labdane diterpene, inhibits both constitutive and inducible nuclear factor-kappa B pathway activation, leading to potentiation of apoptosis, inhibition of invasion, and suppression of osteoclastogenesis.
Berberine modifies cysteine 179 of IkappaBalpha kinase, suppresses nuclear factor-kappaB-regulated antiapoptotic gene products, and potentiates apoptosis.
Evidence that TNF-TNFR1-TRADD-TRAF2-RIP-TAK1-IKK pathway mediates constitutive NF-kappaB activation and proliferation in human head and neck squamous cell carcinoma.
Hepatocyte-specific IKK gamma/NEMO expression determines the degree of liver injury.
The anti-inflammatory effects of selenium are mediated through 15-deoxy-Delta12,14-prostaglandin J2 in macrophages.
Deletion of the N-terminus of IKKgamma induces apoptosis in keratinocytes and impairs the AKT/PTEN signaling pathway.
TRAF6 ubiquitin ligase is essential for RANKL signaling and osteoclast differentiation.
Suberoylanilide hydroxamic acid potentiates apoptosis, inhibits invasion, and abolishes osteoclastogenesis by suppressing nuclear factor-kappaB activation.
Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) suppresses NF-kappaB activation and NF-kappaB-regulated gene products through modulation of p65 and IkappaBalpha kinase activation, leading to potentiation of apoptosis induced by cytokine and chemotherapeutic agents.
Guggulsterone inhibits osteoclastogenesis induced by receptor activator of nuclear factor-kappaB ligand and by tumor cells by suppressing nuclear factor-kappaB activation.
Skin lesion development in a mouse model of incontinentia pigmenti is triggered by NEMO deficiency in epidermal keratinocytes and requires TNF signaling.
Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway.
Curcumin (diferuloylmethane) inhibits constitutive NF-kappaB activation, induces G1/S arrest, suppresses proliferation, and induces apoptosis in mantle cell lymphoma.
N-(4-hydroxyphenyl)retinamide inhibits invasion, suppresses osteoclastogenesis, and potentiates apoptosis through down-regulation of I(kappa)B(alpha) kinase and nuclear factor-kappaB-regulated gene products.
Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products.
Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products.
IKK mediates ischemia-induced neuronal death.
Deletion of IKK2 in hepatocytes does not sensitize these cells to TNF-induced apoptosis but protects from ischemia/reperfusion injury.
Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor.
Guggulsterone inhibits NF-kappaB and IkappaBalpha kinase activation, suppresses expression of anti-apoptotic gene products, and enhances apoptosis.
Protein farnesyltransferase inhibitor (SCH 66336) abolishes NF-kappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NF-kappaB-regulated gene expression and up-regulation of apoptosis.
Glucocorticoid Receptor Counteracts Tumorigenic Activity of Akt in Skin through Interference with the Phosphatidylinositol 3-Kinase Signaling Pathway.
Flavopiridol inhibits NF-kappaB activation induced by various carcinogens and inflammatory agents through inhibition of IkappaBalpha kinase and p65 phosphorylation: abrogation of cyclin D1, cyclooxygenase-2, and matrix metalloprotease-9.
Cyclooxygenase (COX)-2 inhibitor celecoxib abrogates TNF-induced NF-kappa B activation through inhibition of activation of I kappa B alpha kinase and Akt in human non-small cell lung carcinoma: correlation with suppression of COX-2 synthesis.
Cyclooxygenase (COX)-2 inhibitor celecoxib abrogates TNF-induced NF-kappa B activation through inhibition of activation of I kappa B alpha kinase and Akt in human non-small cell lung carcinoma: correlation with suppression of COX-2 synthesis.
Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis.
Adenosine suppresses activation of nuclear factor-kappaB selectively induced by tumor necrosis factor in different cell types.
Impaired NF-kappa B activation and increased production of tumor necrosis factor alpha in transgenic mice expressing keratin K10 in the basal layer of the epidermis.
Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2.
Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2.
Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester.
Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester.
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation.
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation.
Complete reconstitution of human IkappaB kinase (IKK) complex in yeast. Assessment of its stoichiometry and the role of IKKgamma on the complex activity in the absence of stimulation.
Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation.
Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation.
Leflunomide suppresses TNF-induced cellular responses: effects on NF-kappa B, activator protein-1, c-Jun N-terminal protein kinase, and apoptosis.
Leflunomide suppresses TNF-induced cellular responses: effects on NF-kappa B, activator protein-1, c-Jun N-terminal protein kinase, and apoptosis.
De S, Karim F, Kiessu E, Cushing L, Lin LL, Ghandil P, Hoarau C, Casanova JL, Puel A, Rao VR
The Journal of biological chemistry 2018 Sep 28;293(39):15208-15220
The Journal of biological chemistry 2018 Sep 28;293(39):15208-15220
Uncovering inherent cellular plasticity of multiciliated ependyma leading to ventricular wall transformation and hydrocephalus.
Abdi K, Lai CH, Paez-Gonzalez P, Lay M, Pyun J, Kuo CT
Nature communications 2018 Apr 25;9(1):1655
Nature communications 2018 Apr 25;9(1):1655
NFκB signaling in alveolar rhabdomyosarcoma.
Cleary MM, Mansoor A, Settelmeyer T, Ijiri Y, Ladner KJ, Svalina MN, Rubin BP, Guttridge DC, Keller C
Disease models & mechanisms 2017 Sep 1;10(9):1109-1115
Disease models & mechanisms 2017 Sep 1;10(9):1109-1115
Spontaneous Development of Cutaneous Squamous Cell Carcinoma in Mice with Cell-specific Deletion of Inhibitor of κB Kinase 2.
Kirkley KS, Walton KD, Duncan C, Tjalkens RB
Comparative medicine 2017 Oct 1;67(5):407-415
Comparative medicine 2017 Oct 1;67(5):407-415
Inflammation Improves Glucose Homeostasis through IKKβ-XBP1s Interaction.
Liu J, Ibi D, Taniguchi K, Lee J, Herrema H, Akosman B, Mucka P, Salazar Hernandez MA, Uyar MF, Park SW, Karin M, Ozcan U
Cell 2016 Nov 3;167(4):1052-1066.e18
Cell 2016 Nov 3;167(4):1052-1066.e18
HTLV-1 Tax deregulates autophagy by recruiting autophagic molecules into lipid raft microdomains.
Ren T, Takahashi Y, Liu X, Loughran TP, Sun SC, Wang HG, Cheng H
Oncogene 2015 Jan 15;34(3):334-45
Oncogene 2015 Jan 15;34(3):334-45
Role of ferulic acid in the amelioration of ionizing radiation induced inflammation: a murine model.
Das U, Manna K, Sinha M, Datta S, Das DK, Chakraborty A, Ghosh M, Saha KD, Dey S
PloS one 2014;9(5):e97599
PloS one 2014;9(5):e97599
The protein phosphatase 2A regulatory subunit B56γ mediates suppression of T cell receptor (TCR)-induced nuclear factor-κB (NF-κB) activity.
Breuer R, Becker MS, Brechmann M, Mock T, Arnold R, Krammer PH
The Journal of biological chemistry 2014 May 23;289(21):14996-5004
The Journal of biological chemistry 2014 May 23;289(21):14996-5004
T cell receptor signals to NF-κB are transmitted by a cytosolic p62-Bcl10-Malt1-IKK signalosome.
Paul S, Traver MK, Kashyap AK, Washington MA, Latoche JR, Schaefer BC
Science signaling 2014 May 13;7(325):ra45
Science signaling 2014 May 13;7(325):ra45
Noncanonical NF-κB signaling is limited by classical NF-κB activity.
Gray CM, Remouchamps C, McCorkell KA, Solt LA, Dejardin E, Orange JS, May MJ
Science signaling 2014 Feb 4;7(311):ra13
Science signaling 2014 Feb 4;7(311):ra13
Sirtuin-1 (SIRT1) is required for promoting chondrogenic differentiation of mesenchymal stem cells.
Buhrmann C, Busch F, Shayan P, Shakibaei M
The Journal of biological chemistry 2014 Aug 8;289(32):22048-62
The Journal of biological chemistry 2014 Aug 8;289(32):22048-62
Curcumin inhibits tumor growth and angiogenesis in an orthotopic mouse model of human pancreatic cancer.
Bimonte S, Barbieri A, Palma G, Luciano A, Rea D, Arra C
BioMed research international 2013;2013:810423
BioMed research international 2013;2013:810423
TANK-binding kinase 1 (TBK1) controls cell survival through PAI-2/serpinB2 and transglutaminase 2.
Delhase M, Kim SY, Lee H, Naiki-Ito A, Chen Y, Ahn ER, Murata K, Kim SJ, Lautsch N, Kobayashi KS, Shirai T, Karin M, Nakanishi M
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 24;109(4):E177-86
Proceedings of the National Academy of Sciences of the United States of America 2012 Jan 24;109(4):E177-86
IKKβ overexpression leads to pathologic lesions in stratified epithelia and exocrine glands and to tumoral transformation of oral epithelia.
Page A, Cascallana JL, Casanova ML, Navarro M, Alameda JP, Pérez P, Bravo A, Ramírez A
Molecular cancer research : MCR 2011 Oct;9(10):1329-38
Molecular cancer research : MCR 2011 Oct;9(10):1329-38
A novel pentamethoxyflavone down-regulates tumor cell survival and proliferative and angiogenic gene products through inhibition of IκB kinase activation and sensitizes tumor cells to apoptosis by cytokines and chemotherapeutic agents.
Phromnoi K, Reuter S, Sung B, Prasad S, Kannappan R, Yadav VR, Chanmahasathien W, Limtrakul P, Aggarwal BB
Molecular pharmacology 2011 Feb;79(2):279-89
Molecular pharmacology 2011 Feb;79(2):279-89
Escin, a pentacyclic triterpene, chemosensitizes human tumor cells through inhibition of nuclear factor-kappaB signaling pathway.
Harikumar KB, Sung B, Pandey MK, Guha S, Krishnan S, Aggarwal BB
Molecular pharmacology 2010 May;77(5):818-27
Molecular pharmacology 2010 May;77(5):818-27
Targeted deletion of hepatocyte Ikkbeta confers growth advantages.
Koch KS, Maeda S, He G, Karin M, Leffert HL
Biochemical and biophysical research communications 2009 Mar 6;380(2):349-54
Biochemical and biophysical research communications 2009 Mar 6;380(2):349-54
HTLV-1 Tax is a critical lipid raft modulator that hijacks IkappaB kinases to the microdomains for persistent activation of NF-kappaB.
Huang J, Ren T, Guan H, Jiang Y, Cheng H
The Journal of biological chemistry 2009 Mar 6;284(10):6208-17
The Journal of biological chemistry 2009 Mar 6;284(10):6208-17
cIAP1, cIAP2, and XIAP act cooperatively via nonredundant pathways to regulate genotoxic stress-induced nuclear factor-kappaB activation.
Jin HS, Lee DH, Kim DH, Chung JH, Lee SJ, Lee TH
Cancer research 2009 Mar 1;69(5):1782-91
Cancer research 2009 Mar 1;69(5):1782-91
Inhibition of IkappaB kinase-nuclear factor-kappaB signaling pathway by 3,5-bis(2-flurobenzylidene)piperidin-4-one (EF24), a novel monoketone analog of curcumin.
Kasinski AL, Du Y, Thomas SL, Zhao J, Sun SY, Khuri FR, Wang CY, Shoji M, Sun A, Snyder JP, Liotta D, Fu H
Molecular pharmacology 2008 Sep;74(3):654-61
Molecular pharmacology 2008 Sep;74(3):654-61
Coronarin D, a labdane diterpene, inhibits both constitutive and inducible nuclear factor-kappa B pathway activation, leading to potentiation of apoptosis, inhibition of invasion, and suppression of osteoclastogenesis.
Kunnumakkara AB, Ichikawa H, Anand P, Mohankumar CJ, Hema PS, Nair MS, Aggarwal BB
Molecular cancer therapeutics 2008 Oct;7(10):3306-17
Molecular cancer therapeutics 2008 Oct;7(10):3306-17
Berberine modifies cysteine 179 of IkappaBalpha kinase, suppresses nuclear factor-kappaB-regulated antiapoptotic gene products, and potentiates apoptosis.
Pandey MK, Sung B, Kunnumakkara AB, Sethi G, Chaturvedi MM, Aggarwal BB
Cancer research 2008 Jul 1;68(13):5370-9
Cancer research 2008 Jul 1;68(13):5370-9
Evidence that TNF-TNFR1-TRADD-TRAF2-RIP-TAK1-IKK pathway mediates constitutive NF-kappaB activation and proliferation in human head and neck squamous cell carcinoma.
Jackson-Bernitsas DG, Ichikawa H, Takada Y, Myers JN, Lin XL, Darnay BG, Chaturvedi MM, Aggarwal BB
Oncogene 2007 Mar 1;26(10):1385-97
Oncogene 2007 Mar 1;26(10):1385-97
Hepatocyte-specific IKK gamma/NEMO expression determines the degree of liver injury.
Beraza N, Lüdde T, Assmus U, Roskams T, Vander Borght S, Trautwein C
Gastroenterology 2007 Jun;132(7):2504-17
Gastroenterology 2007 Jun;132(7):2504-17
The anti-inflammatory effects of selenium are mediated through 15-deoxy-Delta12,14-prostaglandin J2 in macrophages.
Vunta H, Davis F, Palempalli UD, Bhat D, Arner RJ, Thompson JT, Peterson DG, Reddy CC, Prabhu KS
The Journal of biological chemistry 2007 Jun 22;282(25):17964-73
The Journal of biological chemistry 2007 Jun 22;282(25):17964-73
Deletion of the N-terminus of IKKgamma induces apoptosis in keratinocytes and impairs the AKT/PTEN signaling pathway.
Leis H, Sanchis A, Pérez P
Experimental cell research 2007 Feb 15;313(4):742-52
Experimental cell research 2007 Feb 15;313(4):742-52
TRAF6 ubiquitin ligase is essential for RANKL signaling and osteoclast differentiation.
Lamothe B, Webster WK, Gopinathan A, Besse A, Campos AD, Darnay BG
Biochemical and biophysical research communications 2007 Aug 10;359(4):1044-9
Biochemical and biophysical research communications 2007 Aug 10;359(4):1044-9
Suberoylanilide hydroxamic acid potentiates apoptosis, inhibits invasion, and abolishes osteoclastogenesis by suppressing nuclear factor-kappaB activation.
Takada Y, Gillenwater A, Ichikawa H, Aggarwal BB
The Journal of biological chemistry 2006 Mar 3;281(9):5612-22
The Journal of biological chemistry 2006 Mar 3;281(9):5612-22
Plumbagin (5-hydroxy-2-methyl-1,4-naphthoquinone) suppresses NF-kappaB activation and NF-kappaB-regulated gene products through modulation of p65 and IkappaBalpha kinase activation, leading to potentiation of apoptosis induced by cytokine and chemotherapeutic agents.
Sandur SK, Ichikawa H, Sethi G, Ahn KS, Aggarwal BB
The Journal of biological chemistry 2006 Jun 23;281(25):17023-33
The Journal of biological chemistry 2006 Jun 23;281(25):17023-33
Guggulsterone inhibits osteoclastogenesis induced by receptor activator of nuclear factor-kappaB ligand and by tumor cells by suppressing nuclear factor-kappaB activation.
Ichikawa H, Aggarwal BB
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Jan 15;12(2):662-8
Clinical cancer research : an official journal of the American Association for Cancer Research 2006 Jan 15;12(2):662-8
Skin lesion development in a mouse model of incontinentia pigmenti is triggered by NEMO deficiency in epidermal keratinocytes and requires TNF signaling.
Nenci A, Huth M, Funteh A, Schmidt-Supprian M, Bloch W, Metzger D, Chambon P, Rajewsky K, Krieg T, Haase I, Pasparakis M
Human molecular genetics 2006 Feb 15;15(4):531-42
Human molecular genetics 2006 Feb 15;15(4):531-42
Indirubin enhances tumor necrosis factor-induced apoptosis through modulation of nuclear factor-kappa B signaling pathway.
Sethi G, Ahn KS, Sandur SK, Lin X, Chaturvedi MM, Aggarwal BB
The Journal of biological chemistry 2006 Aug 18;281(33):23425-35
The Journal of biological chemistry 2006 Aug 18;281(33):23425-35
Curcumin (diferuloylmethane) inhibits constitutive NF-kappaB activation, induces G1/S arrest, suppresses proliferation, and induces apoptosis in mantle cell lymphoma.
Shishodia S, Amin HM, Lai R, Aggarwal BB
Biochemical pharmacology 2005 Sep 1;70(5):700-13
Biochemical pharmacology 2005 Sep 1;70(5):700-13
N-(4-hydroxyphenyl)retinamide inhibits invasion, suppresses osteoclastogenesis, and potentiates apoptosis through down-regulation of I(kappa)B(alpha) kinase and nuclear factor-kappaB-regulated gene products.
Shishodia S, Gutierrez AM, Lotan R, Aggarwal BB
Cancer research 2005 Oct 15;65(20):9555-65
Cancer research 2005 Oct 15;65(20):9555-65
Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products.
Ichikawa H, Takada Y, Murakami A, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 1;174(11):7383-92
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 1;174(11):7383-92
Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NF-kappa B-regulated gene products.
Ichikawa H, Takada Y, Murakami A, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 1;174(11):7383-92
Journal of immunology (Baltimore, Md. : 1950) 2005 Jun 1;174(11):7383-92
IKK mediates ischemia-induced neuronal death.
Herrmann O, Baumann B, de Lorenzi R, Muhammad S, Zhang W, Kleesiek J, Malfertheiner M, Köhrmann M, Potrovita I, Maegele I, Beyer C, Burke JR, Hasan MT, Bujard H, Wirth T, Pasparakis M, Schwaninger M
Nature medicine 2005 Dec;11(12):1322-9
Nature medicine 2005 Dec;11(12):1322-9
Deletion of IKK2 in hepatocytes does not sensitize these cells to TNF-induced apoptosis but protects from ischemia/reperfusion injury.
Luedde T, Assmus U, Wüstefeld T, Meyer zu Vilsendorf A, Roskams T, Schmidt-Supprian M, Rajewsky K, Brenner DA, Manns MP, Pasparakis M, Trautwein C
The Journal of clinical investigation 2005 Apr;115(4):849-59
The Journal of clinical investigation 2005 Apr;115(4):849-59
Genetic deletion of glycogen synthase kinase-3beta abrogates activation of IkappaBalpha kinase, JNK, Akt, and p44/p42 MAPK but potentiates apoptosis induced by tumor necrosis factor.
Takada Y, Fang X, Jamaluddin MS, Boyd DD, Aggarwal BB
The Journal of biological chemistry 2004 Sep 17;279(38):39541-54
The Journal of biological chemistry 2004 Sep 17;279(38):39541-54
Guggulsterone inhibits NF-kappaB and IkappaBalpha kinase activation, suppresses expression of anti-apoptotic gene products, and enhances apoptosis.
Shishodia S, Aggarwal BB
The Journal of biological chemistry 2004 Nov 5;279(45):47148-58
The Journal of biological chemistry 2004 Nov 5;279(45):47148-58
Protein farnesyltransferase inhibitor (SCH 66336) abolishes NF-kappaB activation induced by various carcinogens and inflammatory stimuli leading to suppression of NF-kappaB-regulated gene expression and up-regulation of apoptosis.
Takada Y, Khuri FR, Aggarwal BB
The Journal of biological chemistry 2004 Jun 18;279(25):26287-99
The Journal of biological chemistry 2004 Jun 18;279(25):26287-99
Glucocorticoid Receptor Counteracts Tumorigenic Activity of Akt in Skin through Interference with the Phosphatidylinositol 3-Kinase Signaling Pathway.
Leis H, Page A, Ramírez A, Bravo A, Segrelles C, Paramio J, Barettino D, Jorcano JL, Pérez P
Molecular endocrinology (Baltimore, Md.) 2004 Feb;18(2):303-11
Molecular endocrinology (Baltimore, Md.) 2004 Feb;18(2):303-11
Flavopiridol inhibits NF-kappaB activation induced by various carcinogens and inflammatory agents through inhibition of IkappaBalpha kinase and p65 phosphorylation: abrogation of cyclin D1, cyclooxygenase-2, and matrix metalloprotease-9.
Takada Y, Aggarwal BB
The Journal of biological chemistry 2004 Feb 6;279(6):4750-9
The Journal of biological chemistry 2004 Feb 6;279(6):4750-9
Cyclooxygenase (COX)-2 inhibitor celecoxib abrogates TNF-induced NF-kappa B activation through inhibition of activation of I kappa B alpha kinase and Akt in human non-small cell lung carcinoma: correlation with suppression of COX-2 synthesis.
Shishodia S, Koul D, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2011-22
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2011-22
Cyclooxygenase (COX)-2 inhibitor celecoxib abrogates TNF-induced NF-kappa B activation through inhibition of activation of I kappa B alpha kinase and Akt in human non-small cell lung carcinoma: correlation with suppression of COX-2 synthesis.
Shishodia S, Koul D, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2011-22
Journal of immunology (Baltimore, Md. : 1950) 2004 Aug 1;173(3):2011-22
Identification of a p65 peptide that selectively inhibits NF-kappa B activation induced by various inflammatory stimuli and its role in down-regulation of NF-kappaB-mediated gene expression and up-regulation of apoptosis.
Takada Y, Singh S, Aggarwal BB
The Journal of biological chemistry 2004 Apr 9;279(15):15096-104
The Journal of biological chemistry 2004 Apr 9;279(15):15096-104
Adenosine suppresses activation of nuclear factor-kappaB selectively induced by tumor necrosis factor in different cell types.
Majumdar S, Aggarwal BB
Oncogene 2003 Feb 27;22(8):1206-18
Oncogene 2003 Feb 27;22(8):1206-18
Impaired NF-kappa B activation and increased production of tumor necrosis factor alpha in transgenic mice expressing keratin K10 in the basal layer of the epidermis.
Santos M, Perez P, Segrelles C, Ruiz S, Jorcano JL, Paramio JM
The Journal of biological chemistry 2003 Apr 11;278(15):13422-30
The Journal of biological chemistry 2003 Apr 11;278(15):13422-30
Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2.
Anto RJ, Mukhopadhyay A, Shishodia S, Gairola CG, Aggarwal BB
Carcinogenesis 2002 Sep;23(9):1511-8
Carcinogenesis 2002 Sep;23(9):1511-8
Cigarette smoke condensate activates nuclear transcription factor-kappaB through phosphorylation and degradation of IkappaB(alpha): correlation with induction of cyclooxygenase-2.
Anto RJ, Mukhopadhyay A, Shishodia S, Gairola CG, Aggarwal BB
Carcinogenesis 2002 Sep;23(9):1511-8
Carcinogenesis 2002 Sep;23(9):1511-8
Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester.
Majumdar S, Lamothe B, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2002 Mar 15;168(6):2644-51
Journal of immunology (Baltimore, Md. : 1950) 2002 Mar 15;168(6):2644-51
Thalidomide suppresses NF-kappa B activation induced by TNF and H2O2, but not that activated by ceramide, lipopolysaccharides, or phorbol ester.
Majumdar S, Lamothe B, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2002 Mar 15;168(6):2644-51
Journal of immunology (Baltimore, Md. : 1950) 2002 Mar 15;168(6):2644-51
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation.
Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6490-7
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6490-7
Piceatannol inhibits TNF-induced NF-kappaB activation and NF-kappaB-mediated gene expression through suppression of IkappaBalpha kinase and p65 phosphorylation.
Ashikawa K, Majumdar S, Banerjee S, Bharti AC, Shishodia S, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6490-7
Journal of immunology (Baltimore, Md. : 1950) 2002 Dec 1;169(11):6490-7
Complete reconstitution of human IkappaB kinase (IKK) complex in yeast. Assessment of its stoichiometry and the role of IKKgamma on the complex activity in the absence of stimulation.
Miller BS, Zandi E
The Journal of biological chemistry 2001 Sep 28;276(39):36320-6
The Journal of biological chemistry 2001 Sep 28;276(39):36320-6
Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation.
Majumdar S, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2001 Sep 1;167(5):2911-20
Journal of immunology (Baltimore, Md. : 1950) 2001 Sep 1;167(5):2911-20
Methotrexate suppresses NF-kappaB activation through inhibition of IkappaBalpha phosphorylation and degradation.
Majumdar S, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2001 Sep 1;167(5):2911-20
Journal of immunology (Baltimore, Md. : 1950) 2001 Sep 1;167(5):2911-20
Leflunomide suppresses TNF-induced cellular responses: effects on NF-kappa B, activator protein-1, c-Jun N-terminal protein kinase, and apoptosis.
Manna SK, Mukhopadhyay A, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 15;165(10):5962-9
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 15;165(10):5962-9
Leflunomide suppresses TNF-induced cellular responses: effects on NF-kappa B, activator protein-1, c-Jun N-terminal protein kinase, and apoptosis.
Manna SK, Mukhopadhyay A, Aggarwal BB
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 15;165(10):5962-9
Journal of immunology (Baltimore, Md. : 1950) 2000 Nov 15;165(10):5962-9
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Supportive validation
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- Novus Biologicals (provider)
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- Western Blot: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of A) human Daudi, B) HeLa, and C) mouse NIH3T3 lysate probed with IKKbeta antibody at 2 ug/ml.
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- Simple Western: IKK beta Antibody (10AG2) [NB100-56509] - Lane view shows a specific band for IKK beta in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Supportive validation
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- Novus Biologicals (provider)
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- Immunohistochemistry-Paraffin: IKK beta Antibody (10AG2) [NB100-56509] - Human gall bladder probed with IKKbeta antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.
Supportive validation
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- Novus Biologicals (provider)
- Main image
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- Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Intracellular staining of HEK293 cells with 0.5 ug of IKKbeta antibody (red) and 0.5 ug of isotype control antibody (20109, green). Intracellular flow kit was used for this test, and an anti-mouse IgG1 PE conjugated secondary.
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- Experimental details
- Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using Alexa Fluor (R) 647 conjugate of NB100-56509. An intracellular stain was performed on HeLa cells with IKK beta antibody (10AG2) NB100-56509 (blue) and a matched isotype control NBP2-27287 (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. 1 ug of antibody was added to 100 uL of staining buffer and cells were incubated for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis using the Alexa Fluor (R) 488 conjugate of NB100-56509. Staining of IKKbeta in HEK 293 cells using 0.1 ug of Alexa Fluor 488-conjugated antibody. Green histogram represents the isotype control , red represents the IKKbeta antibody.
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: IKK beta Antibody (10AG2) [NB100-56509] - Analysis of PE conjugate of NB100-56509. An intracellular stain was performed on Jurkat cells with IKK Beta antibody (10AG2) NB100-56509PE (blue) and a matched isotype control NBP2-27287AF488 (orange). Cells were fixed with 4% PFA and then permeablized wi
