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Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Immunohistochemistry [2]
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- Product number
- NB600-477 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB600-477, RRID:AB_10002834
- Product name
- Rabbit Polyclonal IKK beta Antibody
- Antibody type
- Polyclonal
- Description
- Delipidation and Defibrination. Control peptide will compete only with the specific reaction of antiserum with the IKKb subunit.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Antigen sequence
Recognition is at the highly conser
ved C-terminal region.- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references IKKβ deficiency in myeloid cells ameliorates Alzheimer's disease-related symptoms and pathology.
Liu Y, Liu X, Hao W, Decker Y, Schomburg R, Fülöp L, Pasparakis M, Menger MD, Fassbender K
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Sep 24;34(39):12982-99
The Journal of neuroscience : the official journal of the Society for Neuroscience 2014 Sep 24;34(39):12982-99
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IKK beta Antibody [NB600-477] - Western blot of anti-IKKBeta antibody reacted with HeLa cell extract. All incubations except color development were performed using TBS supplemented with 0.1% Tween-20 at room temperature. The membrane was blocked in 5% dry milk for 2 h. After washing, a 1:500 dilution of the primary antibody was added to the membrane and incubated for 2 h. Washes with buffer were performed 4 times for 5' each. The western blot was incubated with secondary antibody (HRP Goat-a-Rabbit IgG [H&L]) diluted 1:2,000 for 1h. Washes with TBS preceded color development.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477] - Paraffin-embedded mouse brain sections (IKK beta KO or IKK beta WT) were stained with IKK beta (green). Note: during staining, remember to add 0.1-0.3% Triton-100 in the antibody incubation buffer. See PMID 25253847 for further details regarding the experiemental conditions. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IKK beta Antibody [NB600-477] - Human placenta tissue.
