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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- GTX103138 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX103138, RRID:AB_11171867
- Product name
- MDA5 antibody [N2C1], Internal
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Rabbit
Submitted references Herpes simplex virus 1 tegument protein US11 downmodulates the RLR signaling pathway via direct interaction with RIG-I and MDA-5.
Xing J, Wang S, Lin R, Mossman KL, Zheng C
Journal of virology 2012 Apr;86(7):3528-40
Journal of virology 2012 Apr;86(7):3528-40
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ug of whole cell lysate) A: Raji 7.5% SDS PAGE GTX103138 diluted at 1:1000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) THP-1 whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with MDA5 antibody [N2C1], Internal (GTX103138) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- MDA5 antibody [N2C1], Internal detects MDA5 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MDA5 antibody [N2C1], Internal (GTX103138) diluted at 1:500.
