LS-C345054

antibody from LSBio

Targeting: IFIH1 Hlcd, IDDM19, MDA-5, MDA5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Recommended by provider)

Antibody Data

Product number

LS-C345054

Provider

LSBio

Product name

IFIH1 / MDA5 Antibody (aa2-208) LS-C345054

Antibody type

Polyclonal

Description

Antiserum

Reactivity

Mouse

Host

Rabbit

Storage

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis anti-MDA5 (mouse), pAb (AL180) detecting the cleavage of endogenous mouse MDA5 as an indicator of apoptosis. Method: Cleavage of endogenous Helicard in murine A20 B lymphomas undergoing Fas-mediated apoptosis. A20 cells, either sensitive (S) or resistant (R) to FasL, were treated with 200 ng/ml of FasL for 4 hours and harvested at the indicated times. The cells were lysed and the cell extracts resolved by SDS-PAGE under reducing conditions. After transfer to nitrocellulose the membrane was incubated with anti-MDA5 (mouse), pAb (AL180) at a dilution of 1:1000 for 2 hours. Detection of MDA5 was performed using a peroxidase-conjugated antibody to rabbit Ig (Jackson Lab.) followed by a chemiluminescence detection system. Note: In non-stimulated A20S cells MDA5 has a molecular weight of 140kDa. Upon FasL-induced activation, cleavage products of 120, 41/45, 30 and 28kDa can be detected.