PA5-27848

antibody from Invitrogen Antibodies

Targeting: IFIH1 Hlcd, IDDM19, MDA-5, MDA5

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27848

Provider

Invitrogen Antibodies

Product name

MDA5 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: THP-1, THP-1 (100 ng/ml PMA treatment for 16 hr and 1000 ng/ml LPS treatment for 24 hr).

Concentration

1 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of MDA5 using 30 µg of Raji lysate. Samples were loaded onto a 7.5% SDS-PAGE gel and probed with a MDA5 polyclonal antibody (Product # PA5-27848) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot analysis of MDA5 was performed by separating 30 µg of untreated (–) and treated (+) THP-1 whole cell extracts by 7.5% SDS-PAGE. Proteins were transferred to a membrane and probed with a MDA5 Polyclonal Antibody (Product # PA5-27848) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using MDA5 Polyclonal Antibody (Product # PA5-27848) and a 130 kDa band corresponding to MDA5 was observed upon IFN gamma treatment in HeLa. Whole cell extracts (30 µg lysate) of HeLa (Lane 1), HeLa treated with IFN-gamma (100ng for 72 Hrs) (Lane 2) were electrophoresed using NuPAGE® 4-12 % Bis-Tris gel (Product # NP0321BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (PA5-27848,1:1000 dilution) and detected by Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

MDA5 Polyclonal Antibody detects MDA5 protein at cytoplasm on human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MDA5 Polyclonal Antibody (Product # PA5-27848) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.