AF780
antibody from Novus Biologicals
Targeting: IL1RN
ICIL-1RA, IL-1RN, IL1F3, IL1RA, IRAP, MGC10430
Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- AF780 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Goat Polyclonal IL-1ra/IL-1F3/IL1RN Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects porcine IL-1ra/IL-1F3 in direct ELISAs and Western blots.
- Reactivity
- Porcine
- Host
- Goat
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Submitted references Induction of interleukin-1 in articular cartilage by explantation and cutting.
Gruber J, Vincent TL, Hermansson M, Bolton M, Wait R, Saklatvala J
Arthritis and rheumatism 2004 Aug;50(8):2539-46
Arthritis and rheumatism 2004 Aug;50(8):2539-46
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- IL-1ra/IL-1F3 Inhibition of IL-1 alpha/IL-1F1-dependent Cell Proliferation and Neutral-ization by Porcine IL-1ra/ IL-1F3 Antibody. Recombinant Porcine IL-1ra/ IL-1F3 (Catalog # 780-RA) inhibits Recombinant Porcine IL-1 alpha/IL-1F1 (Catalog # 680-PI) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Porcine IL-1 alpha/ IL-1F1 (75 pg/mL) activity elicited by Recombinant Porcine IL-1ra/ IL-1F3 (0.48 µg/mL) is neutral-ized (green line) by increasing concentrations of Goat Anti-Porcine IL-1ra/IL-1F3 Antigen Affinity-purified Poly-clonal Antibody (Catalog # AF780). The ND50 is typically 3-15 µg/mL in the presence of concanavalin A (1.25 µg/mL).