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LearnMA5-23802
antibody from Invitrogen Antibodies
Targeting: IL1RN
ICIL-1RA, IL-1RN, IL1F3, IL1RA, IRAP, MGC10430
ELISA
Blocking/Neutralizing
Other assayAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Other assay [1]
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- Product number
- MA5-23802 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL1RA Monoclonal Antibody (10309)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In ELISAs, this antibody shows less than 0.2% cross-reactivity with recombinant mouse (rm) IL-1ra and no cross-reactivity with rhIL-1 alpha, rpIL-1 alpha, rmIL-1 alpha, rrIL-1 alpha, rhIL-1 beta, rpIL-1 beta, rmIL-1 beta, rrIL-1 beta, rhIL-1 RI, or rhIL-1 RII. Reconstitute at 0.5 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Antibody clone number
- 10309
- Vial size
- 1 mg
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Neutralization assay of recombinant human IL-1ra/IL-1F3 inhibits recombinant human IL-1a/IL-1F1 induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of recombinant human IL-1a/IL-1F1 (50 pg/mL) activity elicited by recombinant human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of human IL-1ra/IL-1F3 Monoclonal Antibody (Product # MA5-23802). The ND50 is typically 10-20 µg/mL in the presence of concanavalin A (1.25 µg/mL).
