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LearnPA5-21776
antibody from Invitrogen Antibodies
Targeting: IL1RN
ICIL-1RA, IL-1RN, IL1F3, IL1RA, IRAP, MGC10430
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- PA5-21776 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL1RA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: Raji, Neuro2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12. Predicted reactivity: Dog (84%), Pig (82%), Bovine (82%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.86 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Anakinra restores cellular proteostasis by coupling mitochondrial redox balance to autophagy.
Squamous metaplasia amplifies pathologic epithelial-mesenchymal interactions in COPD patients.
van de Veerdonk FL, Renga G, Pariano M, Bellet MM, Servillo G, Fallarino F, De Luca A, Iannitti RG, Piobbico D, Gargaro M, Manni G, D'Onofrio F, Stincardini C, Sforna L, Borghi M, Castelli M, Pieroni S, Oikonomou V, Villella VR, Puccetti M, Giovagnoli S, Galarini R, Barola C, Maiuri L, Della Fazia MA, Cellini B, Talesa VN, Dinarello CA, Costantini C, Romani L
The Journal of clinical investigation 2022 Jan 18;132(2)
The Journal of clinical investigation 2022 Jan 18;132(2)
Squamous metaplasia amplifies pathologic epithelial-mesenchymal interactions in COPD patients.
Araya J, Cambier S, Markovics JA, Wolters P, Jablons D, Hill A, Finkbeiner W, Jones K, Broaddus VC, Sheppard D, Barzcak A, Xiao Y, Erle DJ, Nishimura SL
The Journal of clinical investigation 2007 Nov;117(11):3551-62
The Journal of clinical investigation 2007 Nov;117(11):3551-62
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL-1ra using 30 µg Raji whole cell lysate. Samples were loaded onto a 15% SDS-PAGE gel and probed with an IL-1ra polyclonal antibody (Product # PA5-21776) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IL1RA Polyclonal Antibody detects IL1RN protein by western blot analysis. A. 30 µg Neuro2A whole lysate/extract. B. 30 µg C8D30 whole cell lysate/extract. C. 30 µg NIH-3T3 whole cell lysate/extract. D. 30 µg Raw264.7 whole cell lysate/extract. E. 30 µg C2C12 whole cell lysate/extract.15% SDS-PAGE. IL1RA Polyclonal Antibody (Product # PA5-21776) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IL1RA Polyclonal Antibody detects IL1RN protein by western blot analysis. A. 30 µg Neuro2A whole lysate/extract. B. 30 µg C8D30 whole cell lysate/extract. C. 30 µg NIH-3T3 whole cell lysate/extract. D. 30 µg Raw264.7 whole cell lysate/extract. E. 30 µg C2C12 whole cell lysate/extract.15% SDS-PAGE. IL1RA Polyclonal Antibody (Product # PA5-21776) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IL1RA Polyclonal Antibody detects IL1RN protein by western blot analysis. A. 30 µg PC-12 whole cell lysate/extract.15% SDS-PAGE. IL1RA Polyclonal Antibody (Product # PA5-21776) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IL1RA Polyclonal Antibody detects IL1 Receptor antagonist protein by Western blot analysis. Various whole cell extracts (30 µg) were separated by 15 % SDS-PAGE, and blotted with IL1RA Polyclonal Antibody (Product # PA5-21776) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Knockdown of Interleukin-1 receptor antagonist protein was achieved by transfecting A-431 with Interleukin-1 receptor antagonist protein specific siRNAs (Silencer® select Product # s532292, s532291). Western blot analysis (Fig. a) was performed using Whole cell extracts from the Interleukin-1 receptor antagonist protein knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with IL1RA Polyclonal Antibody (Product # PA5-21776, 1:1000 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Interleukin-1 receptor antagonist protein.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot was performed using Anti-IL1RA Polyclonal Antibody (Product # PA5-21776) and a 19 kDa band corresponding to Interleukin-1 receptor antagonist protein was observed across cell lines tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), SK-O-V3 (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:10,000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of IL-1ra in methanol-fixed HeLa cells using an IL-1ra polyclonal antibody (Product # PA5-21776) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using IL1ra antagonist antibody (Product # PA5-21776) at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded PC3 xenograft, using IL1 Receptor antagonist (Product # PA5-21776) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
