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Learn500-P209BT-1MG
antibody from Invitrogen Antibodies
Targeting: IL1RN
ICIL-1RA, IL-1RN, IL1F3, IL1RA, IRAP, MGC10430
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- 500-P209BT-1MG - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL1RA Polyclonal Antibody, Biotin, PeproTech®
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- AA Sequence of recombinant protein: MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE. Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human IL-1RA. Anti-Human IL-1RA-specific antibody was purified by affinity chromatography and then biotinylated. Sandwich ELISA: To detect Human IL-1RA by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human IL-1RA (500-P209) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-1RA. Western Blot: To detect Human IL-1RA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-1RA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 500-P209BT-1MG will be provided as 2 x 500 µg
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Biotin
- Vial size
- 2 x 500 µg
- Concentration
- 0.1-1.0 mg/mL
- Storage
- -20°C
Submitted references Imbalanced Host Response to SARS-CoV-2 Drives Development of COVID-19.
The Global Phosphorylation Landscape of SARS-CoV-2 Infection.
Blanco-Melo D, Nilsson-Payant BE, Liu WC, Uhl S, Hoagland D, Møller R, Jordan TX, Oishi K, Panis M, Sachs D, Wang TT, Schwartz RE, Lim JK, Albrecht RA, tenOever BR
Cell 2020 May 28;181(5):1036-1045.e9
Cell 2020 May 28;181(5):1036-1045.e9
The Global Phosphorylation Landscape of SARS-CoV-2 Infection.
Bouhaddou M, Memon D, Meyer B, White KM, Rezelj VV, Correa Marrero M, Polacco BJ, Melnyk JE, Ulferts S, Kaake RM, Batra J, Richards AL, Stevenson E, Gordon DE, Rojc A, Obernier K, Fabius JM, Soucheray M, Miorin L, Moreno E, Koh C, Tran QD, Hardy A, Robinot R, Vallet T, Nilsson-Payant BE, Hernandez-Armenta C, Dunham A, Weigang S, Knerr J, Modak M, Quintero D, Zhou Y, Dugourd A, Valdeolivas A, Patil T, Li Q, Hüttenhain R, Cakir M, Muralidharan M, Kim M, Jang G, Tutuncuoglu B, Hiatt J, Guo JZ, Xu J, Bouhaddou S, Mathy CJP, Gaulton A, Manners EJ, Félix E, Shi Y, Goff M, Lim JK, McBride T, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, De Wit E, Leach AR, Kortemme T, Shoichet B, Ott M, Saez-Rodriguez J, tenOever BR, Mullins RD, Fischer ER, Kochs G, Grosse R, García-Sastre A, Vignuzzi M, Johnson JR, Shokat KM, Swaney DL, Beltrao P, Krogan NJ
Cell 2020 Aug 6;182(3):685-712.e19
Cell 2020 Aug 6;182(3):685-712.e19
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human IL-1RA by Western Blot analysis IL1RA Polyclonal Antibody, Biotin (Product # 500-P209BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-1RA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Conjugate
- Biotin
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot: To detect Human IL-1RA by Western Blot analysis IL1RA Polyclonal Antibody, Biotin (Product # 500-P209BT-1MG) can be used at a concentration of 0.1-0.2 µg/mL. When used in conjunction with compatible secondary reagents the detection limit for Recombinant Human IL-1RA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
- Conjugate
- Biotin
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Sandwich ELISA: To detect Human IL-1RA by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.25-1.0 µg/mL of IL1RA Polyclonal Antibody, Biotin (Product # 500-P209BT-1MG) is required. This biotinylated polyclonal antibody, in conjunction with PeproTech IL1RA Polyclonal Antibody (Product # 500-P209-1MG) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human IL-1RA.
- Conjugate
- Biotin
