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LearnPA5-13428
antibody from Invitrogen Antibodies
Targeting: IL1RN
ICIL-1RA, IL-1RN, IL1F3, IL1RA, IRAP, MGC10430
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- PA5-13428 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL1RA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 400 µL
- Concentration
- 1.8 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of IL1RN (arrow) using an IL1RN polyclonal antibody (Product # PA5-13428) in 293 cell lysates (2 µg/lane) either nontransfected (Lane 1) or transiently transfected with the IL1RN gene (Lane 2).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of WiDr cells using an IL1RN polyclonal antibody (Product # PA5-13428) at a dilution of 1:10-50, followed by a fluor-conjugated goat anti-rabbit secondary antibody (green). Nuclei were stained with DAPI (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis in formalin-fixed, paraffin-embedded human colon carcinoma using an IL1RN polyclonal antibody (Product # PA5-13428), followed by HRP-conjugated secondary antibody and DAB staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry analysis of 293 cells using an IL1RN polyclonal antibody (Product # PA5-13428) (right) compared to a negative control cell (left) at a dilution of 1:10-50, followed by a FITC-conjugated goat anti-rabbit antibody
