Antibody data
- Antibody Data
- Antigen structure
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- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- AB-280-NA - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-1ra/IL-1F3 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A or G purified. Detects human IL-1ra/IL-1F3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse IL-1ra/IL-1F3, recombinant rat IL-1ra/IL-1F3, recombinant porcine IL-1ra/IL-1F3, and recombinant equine IL-1ra/IL-1F3 is observed, and less than 1% cross-reactivity with recombinant human (rh) IL-1a, rhIL-1b, rhIL-18, and rhIL-1H1 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
P18510
- Isotype
- IgG
- Vial size
- 1 mg
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- IL-1ra/IL-1F3 Inhibition of IL-1 alpha/IL-1F1-dependent Cell Proliferation and Neutral-ization by Human IL-1ra/IL-1F3 Antibody. Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 alpha/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1ra/IL-1F3 Polyclonal Antibody (Catalog # AB-280-NA). The ND50 is typically 5-10 µg/mL in the presence of concanavalin A (1.25 µg/mL).