AB-280-NA

antibody from R&D Systems

Targeting: IL1RN ICIL-1RA, IL-1RN, IL1F3, IL1RA, IRAP, MGC10430

Western blot (Data presented on provider website)

Blocking/Neutralizing (Supportive data in Antibodypedia)

Antibody Data

Product number

AB-280-NA

Provider

R&D Systems

Product name

Human IL-1ra/IL-1F3 Antibody

Antibody type

Polyclonal

Description

Protein A or G purified. Detects human IL-1ra/IL-1F3 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse IL-1ra/IL-1F3, recombinant rat IL-1ra/IL-1F3, recombinant porcine IL-1ra/IL-1F3, and recombinant equine IL-1ra/IL-1F3 is observed, and less than 1% cross-reactivity with recombinant human (rh) IL-1a, rhIL-1b, rhIL-18, and rhIL-1H1 is observed.

Reactivity

Human

Host

Goat

Conjugate

Unconjugated

Antigen sequence

P18510


Isotype

IgG

Vial size

1 mg

Concentration

LYOPH

Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Blocking/Neutralizing

Supportive validation

Submitted by

R&D Systems (provider)

Main image

Experimental details

IL-1ra/IL-1F3 Inhibition of IL-1 alpha/IL-1F1-dependent Cell Proliferation and Neutral-ization by Human IL-1ra/IL-1F3 Antibody. Recombinant Human IL-1ra/IL-1F3 (Catalog # 280-RA) inhibits Recombinant Human IL-1 alpha/IL-1F1 (Catalog # 200-LA) induced proliferation in the D10.G4.1 mouse helper T cell line in a dose-dependent manner (orange line). Inhibition of Recombinant Human IL-1 alpha/IL-1F1 (50 pg/mL) activity elicited by Recombinant Human IL-1ra/IL-1F3 (50 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-1ra/IL-1F3 Polyclonal Antibody (Catalog # AB-280-NA). The ND₅₀ is typically 5-10 µg/mL in the presence of concanavalin A (1.25 µg/mL).