66043-1-IG

antibody from Invitrogen Antibodies

Targeting: LONP1 hLON, LonHS, PIM1, PRSS15

Antibody data

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Validation data

Product number: 66043-1-IG - Provider product page
Provider: Invitrogen Antibodies
Product name: LONP1 Monoclonal Antibody (1C6C12)
Antibody type: Monoclonal
Antigen: Other
Description: Immunogen sequence: EQNANFLDH YLDVPVDLSK VLFICTANVT DTIPEPLRDR MEMINVSGYV AQEKLAIAER YLVPQARALC GLDESKAKLS SDVLTLLIKQ YCRESGVRNL QKQVEKVLRK SAYKIVSGEA ESVEVTPENL QDFVGKPVFT VERMYDVTPP GVVMGLAWTA MGGSTLFVET SLRRPQDKDA KGDKDGSLEV TGQLGEVMKE SARIAYTFAR AFLMQHAPAN DYLVTSHIHL HVPEGATPKD GPSAGCTIVT ALLSLAMGRP VRQNLAMTGE VSLTGKILPV GGIKEKTIAA KRAGVTCIVL PAENKKDFYD LAAFITEGLE VHFVEHYREI FDIAFPDEQA EALAVER (614-959 aa encoded by BC000235 )
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 1C6C12
Vial size: 150 µL
Concentration: 1.56 mg/mL
Storage: -20°C

LONP1 protein structure - 66043-1-IG shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Various lysates were subjected to SDS PAGE followed by western blot with 66043-1-IG (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Jurkat cells were subjected to SDS PAGE followed by western blot with 66043-1-IG ( LONP1 Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Human heart tissue were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: L02 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66043-1-Ig (LONP1 Antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human heart tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human heart tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human liver tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50 (under 10x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human liver tissue slide using 66043-1-Ig (LONP1 Antibody) at dilution of 1:50 (under 40x lens).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66043-1-IG ( LONP1 Antibody) at dilution of 1:800 (under 10x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry of paraffin-embedded human lung cancer tissue slide using 66043-1-IG ( LONP1 Antibody) at dilution of 1:800 (under 40x lens). heat mediated antigen retrieved with Tris-EDTA buffer (pH 9).