H00004077-M05-100ug

antibody from Novus Biologicals

Targeting: NBR1 1A1-3B, CA125, KIAA0049, M17S2

Western blot (Supportive data in Antibodypedia)

ELISA (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00004077-M05-100ug

Provider

Novus Biologicals

Product name

Mouse Monoclonal NBR1 Antibody

Antibody type

Monoclonal

Description

Ascites. NBR1 - neighbor of BRCA1 gene 1 (5C3)

Reactivity

Human

Host

Mouse

Isotype

IgG

Vial size

100 ug

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NBR1 Antibody (5C3) [H00004077-M05] - Western blot analysis of NBR1 over-expressed 293 cell line, cotransfected with NBR1 Validated Chimera RNAi or non-transfected control. Blot probed with H00004077-M05. GAPDH (36.1 kDa) used as loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: NBR1 Antibody (5C3) [H00004077-M05] - Analysis of NBR1 expression in transfected 293T cell line by NBR1 monoclonal antibody (M05A), clone 5C3. Lane 1: NBR1 transfected lysatE (107.4 KDa). Lane 2: Non-transfected lysate.

ELISA

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Sandwich ELISA: NBR1 Antibody (5C3) [H00004077-M05] - Detection limit for recombinant GST tagged NBR1 is approximately 0.03ng/ml as a capture antibody.