PA5-64993

antibody from Invitrogen Antibodies

Targeting: NBR1 1A1-3B, CA125, KIAA0049, M17S2

Immunocytochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-64993

Provider

Invitrogen Antibodies

Product name

NBR1 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: HEGHHVVDEAP PPVVGAKRLA ARAGKKPLAH YSSLVRVLGS DMKTPEDPAV QSFPLVPCDT DQPQDKPPDW FTSYLETFR Highest antigen sequence identity to the following orthologs - mouse 75%, rat 73%.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.1 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of NBR1 in human cell line A549 shows localization to nucleoplasm and vesicles. Samples were probed using a NBR1 Polyclonal Antibody (Product # PA5-64993).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of NBR1 was performed using 70% confluent log phase A-431 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with NBR1 Polyclonal Antibody (Product # PA5-64993) at 2 µg/mL in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing lysosomal localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.