600-401-854

antibody from Rockland Immunochemicals, Inc.

Targeting: AJUBA JUB, MGC15563

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

600-401-854

Provider

Rockland Immunochemicals, Inc.

Product name

Anti-AJUBA (RABBIT) Antibody - 600-401-854

Antibody type

Polyclonal

Vial size

100 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western blot using Rockland's Affinity Purified anti-Ajuba antibody shows detection of Ajuba-RFP fusion protein in cell lysates (arrow-head).  Lanes correspond to 1) vector only trans-fection, 2) human Ajuba-RFP, 3) mouse Ajuba-RFP, and 4) mock transfection. Approximately 50 µg of each lysate was loaded per lane for SDS-PAGE followed by transfer onto nitrocellulose and reaction with a 1:1,700 dilution of anti-Ajuba antibody.  Detection occurred using a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Rabbit IgG [H&L] (611-132-122) for 45 min at room temperature (800 nm channel, green).  Molecular weight estimation was made by comparison to prestained MW markers (indicated at left, 700 nm channel, red).  IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc.  Other detection systems will yield similar results.

Validation comment

Western Blot