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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- GTX113325 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX113325, RRID:AB_1952586
- Product name
- WWOX antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (50 ?g of whole cell lysate) A: mouse brain 12% SDS PAGE GTX113325 diluted at 1:10000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: Raji 12% SDS PAGE GTX113325 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Rat tissue extract (50 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with WWOX antibody (GTX113325) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of paraformaldehyde-fixed A549, using WWOX(GTX113325) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WWOX antibody immunoprecipitates WWOX protein in IP experiments.IP samples: 293T whole cell extractA. Control with 4 £gg of preimmune Rabbit IgGB. Immunoprecipitation of WWOX protein by 4 £gg WWOX antibody (GTX113325)10 % SDS-PAGEThe immunoprecipitated WWOX protein was detected by WWOX antibody (GTX113325) diluted at 1:500.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WWOX antibody detects WWOX protein at cytoplasm on human placenta by immunohistochemical analysis. Sample: Paraffin-embedded placenta. WWOX antibody (GTX113325) dilution: 1:100.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- WWOX antibody detects WWOX protein at cytosol on mouse skin by immunohistochemical analysis. Sample: Paraffin-embedded mouse skin. WWOX antibody (GTX113325) dilution: 1:500.
