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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PAB1091 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1091, RRID:AB_1136136
- Product name
- WWOX polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to amino acids 89-107 of human WWOX.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Antigen sequence
RLAFTVDDNPTKPTTRQRY- Vial size
- 100 μg
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot detection of WWOX in 30 ug of lysate from the murine fibroblast L-929 cell line. Using WWOX polyclonal antibody at 1 : 5000. Lanes loadded in duplicate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- L-929 cells were stained with both anti WWOX (green 1 : 1000 dilution) and nuclei with DAPI (blue) , followed by staining with secondary antibodies, and subjected to confocal microscopy.
- Validation comment
- Immunofluorescence
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Human skin tissue was formalin-fixed and parafin-embeded. The WWOX polyclonal antibody was diluted 1 : 1000.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
