Antibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
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Validation data
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- Product number
- PA5-47717 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- PLCD3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Reconstitute at 0.2 mg/mL in sterile PBS.
- Reactivity
- Human, Mouse
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 0.2 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of HT-29 human colon adenocarcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH-3T3 mouse embryonic fibroblast cell line. PVDF Membrane was probed with 1 µg/mL of Goat Anti-human/mouse PLC-d3 Antigen Affinity-purified Polyclonal Antibody (Product # PA5-47717) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody. Specific bands were detected for PLC-d3 at approximately 100-110 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLCD3 in HT‚29 human colon adenocarcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH‚3T3 mouse embryonic fibroblast cell line. Samples were incubated in PLCD3 polyclonal antibody (Product # PA5-47717) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. Specific bands were detected for PLC‚δ3 at approximately 100‚110 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of PLCD3 in HT‚29 human colon adenocarcinoma cell line, 293T human embryonic kidney cell line, Jurkat human acute T cell leukemia cell line, and NIH‚3T3 mouse embryonic fibroblast cell line. Samples were incubated in PLCD3 polyclonal antibody (Product # PA5-47717) using a dilution of 1 µg/mL followed by a HRP-conjugated Anti-Goat IgG secondary antibody. Specific bands were detected for PLC‚δ3 at approximately 100‚110 kDa (as indicated). This experiment was conducted under reducing conditions.