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Flow cytometryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Flow cytometry [1]
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- Product number
- IC1935N - Provider product page
- Provider
- R&D Systems
- Product name
- Human/Mouse HIF-1 alpha/HIF1A Alexa Fluor® 700-conjugated Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Recognizes human and mouse HIF-1 alpha in direct ELISA.
- Reactivity
- Human, Mouse
- Host
- Mouse
- Conjugate
- Near infrared dye
- Antigen sequence
Q16665.1- Isotype
- IgG
- Antibody clone number
- 241812
- Vial size
- 100 Tests
- Storage
- Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of HIF-1 alpha in MCF-7 Human Cell Line by Flow Cytometry. MCF-7 human breast cancer cell line treated with CoCl2 was stained with Mouse Anti-Human/Mouse HIF-1 alpha Alexa Fluor® 700-conjugated Monoclonal Antibody (Catalog # IC1935N, filled histogram) or isotype control antibody (Catalog # IC002N, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.
