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ELISAAntibody data
- Antibody Data
- Antigen structure
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- Western blot [1]
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- Product number
- LS-C59282 - Provider product page
- Provider
- LSBio
- Product name
- HIF1A / HIF1 Alpha Antibody LS-C59282
- Antibody type
- Polyclonal
- Description
- Antiserum
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Simian, Xenopus
- Host
- Rabbit
- Storage
- Store at 4°C.
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Supportive validation
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- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Anti-HIF-1alphaHYDROXYP564 Antibody - Western Blot. Western blot of anti-HIF-1alphaHYDROXYP564 antibody shows detection (left panel) of hydroxylated HIF-1alpha in nuclear extracts of A549 cells treated with MG132 (a proteosome inhibitor). Hydroxyproline is not recognized on HIF-1alpha when cells are first treated with DFO, a prolyl hydroxylase inhibitor that prevents HIF hydroxylation. Control staining is shown (right panel) using conventional anti-HIF-1alpha. The asterisk marks a band approximately 110 kD in size corresponding to HIF1-????The primary antibody was used at a 1:1000 dilution in 2% BLOTTO. Personal Communication, L. Neckers and O. Aprelikova, NCI, Bethesda, MD.
